Chemical profile and in vitro antischistosomal activity of Crotalaria madurensis different extracts

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Mohamed M.A.; William S.; Ghareeb M.A.; Botros S.S.

Journal: Scientific Reports

Publisher: Nature Research

Publication Date: 10 July 2026

Volume / Issue: Volume 16 / Issue 1

Article No.: 21615

ISSN: 20452322

DOI: 10.1038/s41598-026-60749-7

Scopus: View on Scopus

PubMed: 42432067

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Mohamed M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, P.O. 30), Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; William S., Parasitology Department, Theodor Bilharz Research Institute, Kornaish El- Nile, P.O. 30), Kornaish El-Nile, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, P.O. 30), Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Botros S.S., Pharmacology Department, Theodor Bilharz Research Institute, (P.O. 30), Kornaish El-Nile, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt

Abstract

Chromatographic isolation of Crotalaria madurensis Wight & Arn leaves aqueous methanol extract availed a new potassium sulfonate acylated flavonol glycosides named quercetin 8-potassium sulfonate 3-O-[2-O-sulfonyl]-β-D-4C1-glucopyranosyl-(1’’’’→3’’’)-4-O-[E- caffeoyl]-β-D-4C1-glucopyranosyl-(1’’’→2’’)-3-O-[E-caffeoyl]-β-D-4C1-glucopyranoside (Crotalamadoside A) (1), along with three known flavonoids identified as quercetin 7-O-neohispredoside (2), 3’,4’-dimethoxy quercetin 3-O-neohispredoside (3) and isoquercetin (6) besides two triterpene saponin compounds named as hedragenin 3-O-β-D-4C1-glucopyranoside (4) and hedragenin 3-O-α-L-1C4−rhamnopyranoside (5). This was in addition, to two cinnamic acid derivatives named as E-caffeic acid 4-O-β-D-4C1−glucopranoside (7) and E-caffeic acid (8). Identification of the isolated compounds relied on their chromatographic properties and spectral data (UV, ESI-MS, 1H NMR, 13C NMR, 1H-1H COSY, HSQC, and HMBC). The antischistosomal activity of the C. madurensis different extracts were assessed in vitro using schistosome worm killing, findings revealed that the aqueous methanol extract was the most effective extract, chromatographic fractionation of this extract revealed significant antischistosomal activity for fractions number V and VI rich in flavonoids and triterpene saponins, respectively. © The Author(s) 2026.

Keywords

Antischistosomal; Cinnamic acid derivatives; Crotalaria madurensis; Good health and well-being; Quercetin; Triterpenes; Animals; Crotalaria; Flavonoids; Plant Extracts; Plant Leaves; flavonoid; plant extract; animal; chemistry; plant leaf

Citation Information

Scopus Citations: 0

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