Characterization and bioactivities of a novel copper–zinc superoxide dismutase from the marine snail Rapana venosa: antioxidant, antimicrobial, and anticancer effects

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Saleh M.A.; Habib M.R.; AbdElGhany S.R.; Abouel-Nour M.F.; Masoud H.M.M.

Journal: Beni-Suef University Journal of Basic and Applied Sciences

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: 9 April 2026

Volume / Issue: Volume 15 / Issue 1

Article No.: 37

ISSN: 23148535

DOI: 10.1186/s43088-026-00759-6

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Saleh M.A., Mansoura University, Al Mansurah, Egypt; Habib M.R., Theodor Bilharz Research Institute, Giza, Egypt; AbdElGhany S.R., Mansoura University, Al Mansurah, Egypt; Abouel-Nour M.F., Mansoura University, Al Mansurah, Egypt; Masoud H.M.M., National Research Centre, Cairo, Egypt

Abstract

Background: Marine organisms, particularly snails, are rich sources of bioactive compounds with potential antioxidant, antimicrobial, and anticancer properties, offering promising avenues for drug discovery. Methods: In this study, Rapana venosa extracts were evaluated for antioxidant enzyme activities and tested for antimicrobial and anticancer effects. Furthermore, a superoxide dismutase (SOD) isoenzyme, designated Rapana venosa superoxide dismutase 1 (RvSOD1), was purified from R. venosa using chromatographic techniques, characterized, and evaluated for its antimicrobial and anticancer activities. Results: The crude R. venosa extract demonstrated substantial antioxidant activity, with measurable catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), and glutathione peroxidase (GPx) activities. It exhibited antimicrobial effects, inhibiting Escherichia coli by 71.2% and Candida albicans by 90.8%. The extract also showed cytotoxicity against human cancer cell lines MCF-7 (breast cancer), MDA-MB-231 (triple-negative breast cancer), and A549 (lung adenocarcinoma). The purified RvSOD1 displayed a specific activity of 600.7 U/mg (7.5-fold purification; 30.4% yield). Characterization indicated that RvSOD1 is a copper–zinc SOD, sensitive to potassium cyanide and activated by Zn2+ and Cu2+, with an optimum pH of 8.0 and an apparent molecular mass of ~ 90 kDa. Functionally, RvSOD1 showed antifungal activity against C. albicans (79.9% inhibition) and antibacterial activity against E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus. In anticancer assays, RvSOD1 was active against MCF-7 and MDA-MB-231 cells but was less effective against A549 cells. Notably, the crude extract was more effective than purified RvSOD1 against all three cell lines, suggesting synergistic effects or additional bioactive compounds. Conclusion: Both the R. venosa extract and the isolated RvSOD1 possess significant antioxidant, antimicrobial, and anticancer properties. These findings highlight their potential as promising multifunctional therapeutic candidates for future pharmaceutical and biomedical applications. © The Author(s) 2026.

Keywords

Antimicrobial; Antioxidant; Antitumor; Characterization; Purification; Rapana venosa; Superoxide dismutase

Citation Information

Scopus Citations: 2

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