GC/MS metabolites profiling of Sarasinula plebeia slug mucin in correlation to its anti-inflammatory activity targeting, Cyclooxygenase 2 via in-vitro and in-vivo approach

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Ibrahim A.M.; Younis I.Y.; Selim N.M.; Fayez A.M.; Daihom B.; Morad M.Y.; Mohsen E.

Journal: Biologia

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: 4 March 2026

Volume / Issue: Volume 81 / Issue 3

Article No.: 83

ISSN: 63088

DOI: 10.1007/s11756-026-02141-4

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Ibrahim A.M., Medical Malacology Department, Theodor Bilharz Research Institute, Giza, Egypt; Younis I.Y., Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Selim N.M., Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Fayez A.M., Department of Pharmacology, School of Life and Medical Sciences, University of Hertfordshire Hosted By Global Foundation, New Administrative Capital, Cairo, 11835, Egypt; Daihom B., Department of Pharmaceutics and Industrial Pharmacy, Cairo University, Kasr El-Aini St., Cairo, 11562, Egypt, College of Pharmacy, The American University in Iraq, Baghdad, Iraq; Morad M.Y., Zoology and Entomology Department, Faculty of Science, Helwan University, Helwan, 11795, Egypt; Mohsen E., Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt

Abstract

Significant advancements have been made in the management and prevention of inflammation over the past few decades. The current study aims to investigate the inflammatory potential of the mucin extracted from the land slug Sarasinula plebeia against oxidative stress and inflammation-related processes. Thirty-six distinct peaks were found by GC–MS analysis of this mucin. Lecithin, n-Hexadecanoic acid, 5-Hydroxymethylfurfural, oleic acid ethyl ester, 9,12-Octadecadienoic acid, methyl ester, and lastly, hyoleic safflower oil, are the main peaks. The mucin reacts more selectively toward COX-1 with IC50 22.17 ± 0.63 µg ml−1 compared to Celecoxib IC50 21.27 ± 0.604 µg ml −1, but it exhibited a lower activity towards COX-2 with IC50 7.905 ± 0.32 µg ml−1 as compared to Celecoxib IC50 2.59 ± 0.126 µg ml−1. In this model, neither slug gel nor celecoxib altered systemic oxidative‐stress biomarkers (serum SOD, MDA). Biochemical analysis showed that the higher dose of slug gel reduced serum prostaglandin E2 (PGE2) and tumor necrosis factor-alpha (TNF-α) levels. The present results indicated that both 250 mg and 500 mg concentrations of the slug preparation topical gel significantly reduced rat paw edema, demonstrating an anti-inflammatory effect similar to that of celecoxib 250 mg gel. This potential was revealed by the paw edema diameter test and the measurement of anti-inflammatory markers, PGE2 and TNF-α. In conclusion, S. plebeia mucin has high bioactive capacities and that its crude extract exhibited a good range of anti-inflammatory action, making it valuable for the pharmaceutical business. © Plant Science and Biodiversity Centre, Slovak Academy of Sciences (SAS), Institute of Zoology, Slovak Academy of Sciences (SAS), Institute of Molecular Biology, Slovak Academy of Sciences (SAS) 2026.

Keywords

Anti-collagenase activity; Anti-inflammatory; HS-SPME; Silylation; Slug mucus

Citation Information

Scopus Citations: 0

For comprehensive information about the Theodor Bilharz Research Institute (TBRI), its institutional activities, scientific and research achievements, clinical and hospital services, and the diverse expertise offered through its 22 specialized research and clinical departments, as well as opportunities for professional training, specialized workshops, and scientific conferences, readers are invited to visit the Institute’s official website.

The website provides regularly updated information on the Institute’s latest news, research activities, scientific initiatives, clinical services, institutional programs, and academic and professional opportunities.

English Website: https://www.tbri.sci.eg/en/

Arabic Website: https://www.tbri.sci.eg/ar/

Prepared and Uploaded by:

Abdalla F. Abdalla

Electronic Portal Unit

Electronic Portal Unit

Popular Posts

Transcriptome analysis for Schistosoma vector snail’s Oncomelania hupensis exposed to silver nanoparticles

Image
Bibliographic Information Authors: Zayed K.M.; Zhou X.-N. Journal: Journal of Parasitic Diseases Publisher: Springer Publication Date: 3 September 2026 ISSN: 9717196 DOI: 10.1007/s12639-026-01986-5 Scopus: View on Scopus Document Type: Article Authors and Affiliations Zayed K.M., Medical Malacology Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt, National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention, Shanghai, 200025, China, NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research On Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China; Zhou X.-N., National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention, Shanghai, 200025, China, NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Res...
Read more

Interpretation of liver stiffness measurement in patients with mixed liver disease etiologies

Image
Bibliographic Information Authors: El Ray A.; El Ghannam M.; Rautou P.E. Journal: Arab Journal of Gastroenterology Publisher: Elsevier Ltd Publication Date: August 2026 ISSN: 16871979 DOI: 10.1016/j.ajg.2026.05.002 Scopus: View on Scopus PubMed: 42362465 Document Type: Letter Authors and Affiliations El Ray A., Theodor Bilharz Research Institute, Hepatogastroenterology Department, Giza, Egypt, Faculty of Medicine, October 6 University, Egypt; El Ghannam M., Theodor Bilharz Research Institute, Hepatogastroenterology Department, Giza, Egypt; Rautou P.E., Université Paris-Cité, Inserm, Centre de recherche sur l’inflammation, UMR 1149, Paris, France, AP-HP, Hôpital Beaujon, Service d’Hépatologie, DMU DIGEST, Centre de Référence des Maladies Vasculaires du Foie, FILFOIE, ERN RARE-LIVER, Clichy, France Abstract Vibration-controlled transient elastography (VCTE)–based liver stiffness measurement (LSM) is widely used for non-invasive assessment of liver fibrosis and p...
Read more

Multifunctional polycaprolactone-based composite fibers with icariin and glutathione for effective repair of critical-sized bone defects

Image
Bibliographic Information Authors: Yessuf A.M.; Zhang Y.; Sharma B.P.; Wang Y.; Kassa T.S.; Qadir A.; Jinqiu Y.; Mohideen M.M.; Salama A.M.; Wang W.; Zhang Q.; Liu Y. Journal: BMEMat Publisher: John Wiley and Sons Inc Publication Date: 30 July 2026 ISSN: 27517438 DOI: 10.1002/bmm2.70115 Scopus: View on Scopus Document Type: Article Access: All Open Access; Gold Open Access Authors and Affiliations Yessuf A.M., Key Laboratory of Advanced Functional Polymer Composites, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, China; Zhang Y., Henan Provincial Orthopedic Hospital, Henan, Zhengzhou, China; Sharma B.P., Key Laboratory of Electrochemical Process and Technology of Materials, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, China; Wang Y., Beijing University of Chinese Medicine, Beijing, China; Kassa T.S., Key Laboratory of Advanced Functional Polymer Composites, Col...
Read more

Holothuria arenicola Extract-Loaded Polycaprolactone Nanocapsules Attenuate Bile Duct Ligation-Induced Acute Liver Injury

Image
Bibliographic Information Authors: Mansour E.M.; Shemis M.; Fahmy S.R.; Amer M.A.; Mohamed A.S. Journal: Current Nanomedicine Publisher: Bentham Science Publishers Publication Date: September 2026 Volume / Issue: Volume 16 / Issue 5 Pages: 771–786 ISSN: 24681873 DOI: 10.2174/0124681873401484250924125945 Scopus: View on Scopus Document Type: Article Authors and Affiliations Mansour E.M., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt; Shemis M., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt; Fahmy S.R., Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt; Amer M.A., Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt; Mohamed A.S., Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt Abstract Introduction: Cholestatic liver diseases are characterized by jaundice and cholestasis with various conseq...
Read more

Mytilus edulis-mediated green synthesis of selenium nanoparticles with antimicrobial and molluscicidal applications

Image
Bibliographic Information Authors: Dokmak H.-A.A.; AlSedawy M.; Ramadan M.A.; Amin B.H.; Faid A.H.; Abdelmaksoud H.F.; El-Sayed S.M. Journal: Scientific Reports Publisher: Nature Research Publication Date: 7 September 2026 Volume / Issue: Volume 16 / Issue 1 Article No.: 27918 ISSN: 20452322 DOI: 10.1038/s41598-026-65860-3 Scopus: View on Scopus PubMed: 42706305 Document Type: Article Access: All Open Access; Gold Open Access; Green Open Access Authors and Affiliations Dokmak H.-A.A., Department of Medical Malacology, Theodor Bilharz Research Institute, Giza, Egypt; AlSedawy M., Microbiology and Immunology Department, Faculty of Pharmacy (For Boys), Al-Azhar University, Cairo, Egypt; Ramadan M.A., Department of Laser Application in Metrology, Photochemistry and Agriculture, National Institute of Laser Enhanced Science (NILES) Cairo University (CU), Giza, Egypt; Amin B.H., The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, 11787,...
Read more

Microbial levan potentiates hepatic retention and antitumor activity of a PEGylated benzimidazole–curcumin nanocomplex through TLR2–FXR/FGF15-associated immunometabolic remodeling in experimental liver cancer

Image
Bibliographic Information Authors: Shawky H.; Ibrahim N.E.; Amer M.N.; Al-Ashmawy A.A.K.; Hammam O.A.; Fayed D.B.; Maghraby A.S. Journal: Scientific Reports Publisher: Nature Research Publication Date: 12 August 2026 Volume / Issue: Volume 16 / Issue 1 Article No.: 25094 ISSN: 20452322 DOI: 10.1038/s41598-026-64576-8 Scopus: View on Scopus PubMed: 42581056 Document Type: Article Access: All Open Access; Gold Open Access; Green Open Access Authors and Affiliations Shawky H., Therapeutic Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Cairo, 12622, Egypt; Ibrahim N.E., Microbial Biotechnology Department, Biotechnology Research Institute, National Research Centre, Dokki, Cairo, 12622, Egypt; Amer M.N., Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Cairo, 12622, Egypt; Al-Ashmawy A.A.K., Therapeutic Chem...
Read more

Variable clinical presentations of pulmonary hydatid cysts: a four-case series from a single center in United Arab Emirates, non-endemic region

Image
Bibliographic Information Authors: Yehya M.; Abdallah M.F.H.; Ajeka S.; Al-Masalmeh F.H.; Albaghdadi M.O.J. Journal: Journal of Surgical Case Reports Publisher: Oxford University Press Publication Date: 29 July 2026 Volume / Issue: Volume 2026 / Issue 7 Article No.: rjag658 ISSN: 20428812 DOI: 10.1093/jscr/rjag658 Scopus: View on Scopus Document Type: Article Access: All Open Access; Gold Open Access; Green Open Access Authors and Affiliations Yehya M., Department of Thoracic Surgery, SAQR Hospital, Al Juwais Al Kadi St, Ras Al-Khaimah, United Arab Emirates; Abdallah M.F.H., Department of Radiology, SAQR Hospital, Al Juwais Al Kadi St, Ras Al-Khaimah, United Arab Emirates, Theodor Bilharz Research Institute, Mahad Al Abhas Al bahari St, Kornish Al Nile, Warraq Al Arab, Al Warak, Governorate, Giza, 3863341, Egypt; Ajeka S., Department of General Surgery, SAQR Hospital, Al Juwais Al Kadi St, Ras Al-Khaimah, United Arab Emirates; Al-Masalmeh F.H., Department of ...
Read more