LC–MS/MS Profiling of Algerian Zizyphus lotus Extracts and their in vitro Antioxidant Activity: A Comparative Study; [Cezayir Zizyphus lotus Özütlerinin LC–MS/MS Profillemesi ve Bunların in vitro Antioksidan Aktivitesi: Karşılaştırmalı Bir Çalışma]

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Guenane H.; Bakchiche B.; Erenler R.; Yildiz I.; Ghareeb M.A.

Journal: Commagene Journal of Biology

Publisher: ABADER (Adiyaman Bilimsel Arastirmalar Dernegi)

Publication Date: 18 September 2025

Volume / Issue: Volume 9 / Issue 2

Pages: 137–144

ISSN: 2602456X

DOI: 10.31594/commagene.1654147

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Guenane H., Laboratory of Biological and Agricultural Sciences (LBAS), Amar Telidji University, Laghouat, 03000, Algeria; Bakchiche B., Laboratory of Biological and Agricultural Sciences (LBAS), Amar Telidji University, Laghouat, 03000, Algeria; Erenler R., Department of Chemistry, Faculty of Arts and Sciences, Gaziosmanpasa University, Tokat, Tokat, 60240, Turkey; Yildiz I., Department of Chemistry, Faculty of Arts and Sciences, Gaziosmanpasa University, Tokat, Tokat, 60240, Turkey; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt

Abstract

In this study, it was aimed to determine antioxidant activities and chemical composition of leaf and fruit extracts of Zizyphus lotus which has been used traditionally in folk medicine of Algeria. Total phenolic content (TPC) and total flavonoid content (TFC) were determined by the colorimetric assay, respectively. The extracts were analyzed by LC–MS/MS. Furthermore, the antioxidant potential of various extracts was evaluated using different antioxidant assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, 2,2-azinobis-ethylbenzothiozoline-6-sulphonic acid (ABTS), and Phenanthroline activity using UV spectrophotometer. The quantitative analysis of polyphenols and flavonoids content showed that the leaves have the highest amount (279.07±18.84 mg GAE/g E and 132.74±27.74 mg ER/g E, respectively). The highest antioxidant activity was observed in the leaves with the value of 24.10±3.49 µg/mL, 9.365 ± 0.532 µg/mL, 0.19±0.093, 0.636±0.033 mg AAE/g, and 12.11±0.81 µg/mL for DPPH, ABTS, total antioxidant capacity, FRAP and Phenanthroline activity, respectively. The LC–MS/MS analysis of leaves reveals the presence the major compound, rutin (24392.59 mg/g), when fruits contain hesperidin (311.70 mg/g) as a major compound. This research suggested that these medicinal plants possess a significant antioxidant potential, are an important source of natural antioxidants, and can be effectively used in treating oxidative stress disorders. © 2025 ABADER (Adiyaman Bilimsel Arastirmalar Dernegi). All rights reserved.

Keywords

antioxidant potential; LC–MS/MS analysis; total phenolics; Zizyphus lotus

Citation Information

Scopus Citations: 1

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