Evaluation of the therapeutic effect of M. recutita plant extract against experimental giardiasis

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Mohamed W.A.; El-Sayed R.; Abdelmaksod H.F.; Farah M.A.

Journal: Iraqi Journal of Veterinary Sciences

Publisher: University of Mosul - College of Veterinary Medicine

Publication Date: 5 September 2025

Volume / Issue: Volume 39 / Issue 4

Pages: 835–846

ISSN: 16073894

DOI: 10.33899/ijvs.2025.157756.4147

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Mohamed W.A., Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Egypt; El-Sayed R., Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Egypt; Abdelmaksod H.F., Theodor Bilharz Research Institute, Cairo, Egypt; Farah M.A., Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Egypt

Abstract

Giardia lamblia (G. lamblia) is a common pathogenic protozoan that frequently causes giardiasis, leading to steatorrhea; management of giardiasis is difficult, and the need to find alternative natural products is needed to avoid the adverse effects of chemotherapeutics. Therefore, the present investigation aimed to assess the impacts of M. recutita plant extract in the treatment of experimental giardiasis and evaluate its effect using different parameters of assessment. Mice were classified into: G1: non-infected, non-managed; G2: infected, non-managed; G3: infected and managed with metronidazole; G4: infected and managed with n-hexane extract of M. recutita at 50 % concentration; G5: infected and treated with n-hexane extract of M. recutita at 100 % concentration; G6: infected and managed with n-butanol extract of M. recutita at 50 % concentration; and G7: infected and managed with n-butanol extract of M. recutita at 100 % concentration. The therapeutic efficacy was evaluated by parasitological (cyst and trophozoite count), histopathological, immunohistochemical (Caspase-3), histochemical (PAS staining), and oxidative stress markers (SOD and MDA) parameters. The highest therapeutic outcome was achieved with G3 and G5, showing significant reduction with a percentage of 13.6±3.51 and 13.8±1.92, respectively, and an apparent amendment of pathological insults, immunohistochemical staining, and immunological markers. Results of the present research proved that the M. recutita plant extract has a promising therapeutic effect against Giardia lamblia. The plant extract from M. recutita is readily available and helps prevent the negative side effects of chemotherapy medications. © Authors, 2025, College of Veterinary Medicine, University of Mosul.

Keywords

Caspase-3; Giardia lamblia; Histopathological; M. recutita; caspase 3; essential oil; hexane; malonaldehyde; Matricaria chamomilla extract; metronidazole; plant extract; superoxide dismutase; animal experiment; animal tissue; Article; controlled study; gas chromatography; Giardia intestinalis; giardiasis; histopathology; immunohistochemistry; male; mass spectrometry; medicinal plant; microscopy; molecular weight; mouse; nonhuman; oxidative stress; physiological stress; steatorrhea; therapy effect; treatment outcome; trophozoite; veterinary medicine

Citation Information

Scopus Citations: 1

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