2-Hydroxychalcone attenuates airway inflammation, oxidative stress, and NF-κB activation in ovalbumin-induced asthma model

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdelmawgood I.A.; Kotb M.A.; Waleed Ebeed B.; Samy Hassan Eltras H.; Mahmoud Badr A.; Mahana N.A.; Saber Mohamed A.; Goda L.; Lotfy Khalaf M.; Sayed A.M.; Zahran R.G.; Abdelazeem M.S.; Eltaweel S.H.; Ali Ramadan S.; Sharaf B.; Adel A.; Ashry H.; Hesham Abdelfattah H.

Journal: Journal of Asthma

Publisher: Taylor & Francis Group LLC Philadelphia

Publication Date: 4 July 2025

Volume / Issue: Volume 62 / Issue 11

Pages: 1880–1892

ISSN: 2770903

DOI: 10.1080/02770903.2025.2526384

Scopus: View on Scopus

PubMed: 40577600

Document Type: Article

Authors and Affiliations

Abdelmawgood I.A., Zoology Department, Faculty of Science, Cairo University, Giza, Egypt; Kotb M.A., Zoology Department, Faculty of Science, Cairo University, Giza, Egypt; Waleed Ebeed B., Zoology Department, Faculty of Science, Cairo University, Giza, Egypt; Samy Hassan Eltras H., Faculty of Pharmacy, Tanta University, Tanta, Egypt; Mahmoud Badr A., Zoology Department, Faculty of Science, Cairo University, Giza, Egypt; Mahana N.A., Zoology Department, Faculty of Science, Cairo University, Giza, Egypt; Saber Mohamed A., Zoology Department, Faculty of Science, Cairo University, Giza, Egypt; Goda L., Pathology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Lotfy Khalaf M., Zoology Department, Faculty of Science, Cairo University, Giza, Egypt; Sayed A.M., Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt; Zahran R.G., Biochemistry Branch, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt; Abdelazeem M.S., Biochemistry Branch, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt; Eltaweel S.H., Biochemistry Branch, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt; Ali Ramadan S., Department of Molecular Biotechnology, Faculty of Science, Helwan University, Cairo, Egypt; Sharaf B., Department of Molecular Biotechnology, Faculty of Science, Helwan University, Cairo, Egypt; Adel A., Biochemistry Branch, Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt; Ashry H., Basic Sciences Department, Saint Petersburg University in Cairo, Cairo, Egypt; Hesham Abdelfattah H., Zoology Department, Faculty of Science, Cairo University, Giza, Egypt

Abstract

Background: Asthma is a pulmonary disorder marked by limited lung function and a noticeable infiltrate of inflammatory cells. Chalcones are commonly found in food plants and are regarded as precursors of flavonoids. Moreover, they can also be synthesized by chemical processes in controlled laboratory conditions. 2-Hydroxychalcone (2HC) has been associated with several biological functions, such as antioxidant, anti-inflammatory, antidiabetic, and anticancer properties. However, we have yet to study its potential for treating asthma. This study aimed to investigate the inhibitory effects of 2HC on allergic asthma. Methods: We established a mouse model of asthma by subjecting BALB/c mice to ovalbumin (OVA) exposure and sensitization. We conducted a study to examine the impact of 2HC on airway inflammation, the infiltration of inflammatory cells, oxidative stress, excessive mucus production, immunoglobulin E (IgE) levels, and the production of IL-4 and IL-1β. In addition, NF-κB expression was quantified using ELISA and immunohistochemistry (IHC). Results: The administration of 2HC resulted in a considerable reduction in lung oxidative stress, infiltration of inflammatory cells, levels of IgE, IL-4, and IL-1β, production of mucus, and activation of NF-κB. Conclusion: Our findings showed that 2HC is anti-inflammatory and antioxidant, suggesting that it may be useful in treating asthma by blocking NF-κB activation. © 2025 Taylor & Francis Group, LLC.

Keywords

2-hydroxychalcone; Allergic asthma; inflammation; NF-κB; ovalbumin; oxidative stress; Animals; Asthma; Bronchoalveolar Lavage Fluid; Chalcones; Disease Models, Animal; Female; Immunoglobulin E; Interleukin-1beta; Interleukin-4; Lung; Mice; Mice, Inbred BALB C; NF-kappa B; 2 hydroxychalcone; chalcone derivative; immunoglobulin enhancer binding protein; interleukin 1beta; interleukin 4; unclassified drug; animal experiment; animal model; animal tissue; Article; cell count; cell infiltration; controlled study; drug effect; enzyme linked immunosorbent assay; histopathology; immunohistochemistry; inflammatory cell; lung weight; mouse; mucus secretion; nonhuman; protein expression; respiratory tract inflammation; animal; Bagg albino mouse; blood; cytology; disease model; drug therapy; immunology; metabolism; pathology

Citation Information

Scopus Citations: 3

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