Identification and exploration of anticancer activity of novel peptides isolated from the edible bivalve Callista chione in hepatic and colon cancer cell lines

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Hussein A.A.A.; Okasha H.; ElZallat M.; Ghoname S.I.; Habib M.R.; Hammam O.A.; El-Dabaa E.; Salem M.B.

Journal: Toxicology Reports

Publisher: Elsevier Inc.

Publication Date: June 2025

Volume / Issue: Volume 14

Article No.: 101915

ISSN: 22147500

DOI: 10.1016/j.toxrep.2025.101915

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Hussein A.A.A., Departments of Medical Malacology, Theodor Bilharz Research Institute, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Okasha H., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; ElZallat M., Immunology Department, Theodor Bilharz Research Institute, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Ghoname S.I., Departments of Medical Malacology, Theodor Bilharz Research Institute, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Habib M.R., Departments of Medical Malacology, Theodor Bilharz Research Institute, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Hammam O.A., Pathology Department, Theodor Bilharz Research Institute, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; El-Dabaa E., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Salem M.B., Pharmacology Department, Theodor Bilharz Research Institute, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt

Abstract

Background: The requirement for relevant and safe drugs for cancer treatment is considered a challenge. Recently, marine isolated compounds with various therapeutic targets have attracted many researchers. Aim: Isolation and identification of potential anticancer peptides from edible Callista chione soft tissues. Methodology: C. chione specimens were collected and peptides were extracted, purified with FPLC, and tested on normal (hepatocytes and VERO) and cancer (HepG2, and HT-29) cells. Bioactive fractions were tested by tandem mass spectrometry. Results: Five different fractions were purified according to ionic charges and two fractions (4 and 5) showed a potent anticancer activity with a total anticancer score threshold of ≥ 0.5, and hydrophilicity mean of 1.75 that related to stability and solubility. The apoptotic and autophagy-related markers were significantly up-regulated in both HepG2 and HT-29 cells treated with IC50 of bioactive peptides’ fractions 4 and 5, explaining their underlying mechanism of action. Conclusion: Natural source peptides derived from the soft tissue of C. chione could be exploited for the treatment of cancers and a deep in silico study will be performed for further investigation and deep function identification. © 2025 The Authors

Keywords

Anticancer; Apoptosis; Autophagy; Bioactive peptides; FGF19-FGFR4 axis; FPLC; Marine sources; beclin 1; caspase 3; peptide; animal tissue; antineoplastic activity; Article; autophagy (cellular); bivalve; bivalve Callista chione; cell proliferation; cell viability; colon cancer cell line; controlled study; cytotoxicity; enzyme linked immunosorbent assay; exploratory behavior; flow cytometry; gene expression; human; human cell; hydrophilicity; hydrophobicity; IC50; liver cancer cell line; nonhuman; particle size; protein expression; proteomics; sequence analysis; tandem mass spectrometry

Citation Information

Scopus Citations: 6

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