GC-MS AND LC-MS ANALYSIS UNVEIL THE MULTIFACETED BIOACTIVITY OF EREMINA DESERTORUM SNAIL MUCUS: ANTIMICROBIAL, ANTIOXIDANT, WOUND REGENERATION, AND ANTICANCER EFFICACY AUGMENTED BY MOLECULAR DOCKING INSIGHTS

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Ali S.E.; Ibrahim A.M.; Atta S.A.; Hamada M.A.; Gamil I.S.; El-Sayed H.; Morad M.Y.

Journal: Bulletin of Pharmaceutical Sciences. Assiut

Publisher: Assiut University

Publication Date: 24 June 2025

Volume / Issue: Volume 48 / Issue 2

Pages: 1151–1171

ISSN: 11100052

DOI: 10.21608/BFSA.2025.367074.2471

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Ali S.E., Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt; Ibrahim A.M., Medical Malacology Department, Theodor Bilharz Research Institute, Giza, Egypt; Atta S.A., Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Hamada M.A., Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo, Egypt; Gamil I.S., Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt; El-Sayed H., Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo, Egypt; Morad M.Y., Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt

Abstract

The current investigation evaluated the biological efficiency of E. desertorum snail mucus for antimicrobial, antioxidant, wound healing, and antitumor activities. GC-MS analysis identified major compounds, including triphenylmethane, palmitic acid, and pentadecanoic acid. LC-MS revealed key metabolites such as betaine, thiamine, leucine, and tryptophan. The mucus showed low antibacterial but moderate antifungal effects. It exhibited strong antioxidant activity, scavenging DPPH free radicals with an IC50 of 8.88 µg/mL. Wound healing was enhanced in mice treated with mucus, and a scratch wound assay confirmed its role in accelerating healing. The mucus significantly inhibited hepatic (HepG-2) and colorectal (CaCo-2) cancer cell proliferation, with IC50 values of 3.6 mg/mL and 0.2 µg/mL, respectively. Molecular docking suggested interactions between mucus compounds and microbial or cancer cell receptors. These findings highlight E. desertorum mucus as a promising natural source for antimicrobial, antioxidant, wound healing, and anticancer applications. © 2025, Assiut University. All rights reserved.

Keywords

antimicrobial; Caco-2cell lines; Eremina desertorum; HepG-2 cell line; molecular docking; wound healing; 5 aminovaleric acid; animal extract; antiinfective agent; betaine; Eremina desertorum extract; gentamicin; glycolic acid; leucine; palmitic acid; pentadecanoic acid; thiamine; triphenylmethane; tryptophan; unclassified drug; animal experiment; animal model; animal tissue; antibacterial activity; antifungal activity; antimicrobial activity; antineoplastic activity; antioxidant activity; Article; bacterial strain; biological activity; Caco-2 cell line; cancer inhibition; cell proliferation; computer model; controlled study; drug efficacy; Hep-G2 cell line; human; human cell; IC50; liquid chromatography-mass spectrometry; male; mass fragmentography; minimum inhibitory concentration; mouse; mucus; nonhuman; scratch wound; snail

Citation Information

Scopus Citations: 0

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