Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Hassan Z.R.; Mahmoud E.M.; Shaaban Y.M.; Elshora S.Z.; Salama D.E.A.; Abdelgalil R.M.; Koullah M.T.; Seliem N.; Sedira M.H.; Mohamed S.A.; Ali Mohammad Etewa M.; Nagdy Mahmoud Mousa S.; Shaban Abdelmonsef Abdelmaksoud A.; Attia Atta S.; Zakaria M.; Emam S.R.; Hassan M.; Khamis G.

Journal: Acta Parasitologica

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: 2 June 2025

Volume / Issue: Volume 70 / Issue 3

Article No.: 119

ISSN: 12302821

DOI: 10.1007/s11686-025-01045-8

Scopus: View on Scopus

PubMed: 40455365

Document Type: Article

Access: All Open Access; Green Open Access; Hybrid Gold Open Access

Authors and Affiliations

Hassan Z.R., Departments of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt, Departments of Parasitology, Benha National University (BNU) Qalyubia, Obour, Egypt; Mahmoud E.M., Departments of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Shaaban Y.M., Departments of Parasitology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Elshora S.Z., Department of Histology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Salama D.E.A., Department of Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt, Department of Pathology, School of Medicine, Badr University in Cairo (BUC), Cairo, Egypt; Abdelgalil R.M., Department of Anatomy and Embryology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Koullah M.T., Department of Anatomy and Embryology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Seliem N., Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt, Department of Medical Biochemistry and Molecular biology, School of Medicine, Badr University in Cairo (BUC), Cairo, Egypt; Sedira M.H., Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Mohamed S.A., Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Ali Mohammad Etewa M., Department of Physiology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Nagdy Mahmoud Mousa S., Department of Physiology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; Shaban Abdelmonsef Abdelmaksoud A., Department of Anatomy and Physiology, College of Medicine, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia, Department of Medical Physiology, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt; Attia Atta S., Department of Immunology, Theodor Bilharz Research Institute, Cairo, Egypt; Zakaria M., Department of Educational Central Lab, Women College, Ain Shams University, Cairo, Egypt, Department of Pharmaceutical Production Technology, Faculty of Applied Health Science Technology, October Technological Univerisity, Giza, Egypt; Emam S.R., Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Hassan M., Department of Laser Application in Metrology, Photochemistry and Agriculture (LAMPA), National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, Egypt; Khamis G., Department of Laser Application in Metrology, Photochemistry and Agriculture (LAMPA), National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, Egypt

Abstract

Background: Trichinosis is a severe parasitic disease with a wide distribution and potential to affect humans. Available chemotherapeutic agents exhibit limited efficacy and are associated with numerous side effects. This study evaluated the ameliorative effects of blue LED irradiated and non-irradiated fenugreek seed extracts on experimental trichinosis. Methods: Eighty-four mice were divided into seven groups; each further subdivided into intestinal and muscular phases (six mice per subgroup): non-infected non-treated controls, infected non-treated controls, infected albendazole-treated, infected non-irradiated fenugreek extract-treated, infected blue LED irradiated fenugreek extract-treated, infected non-irradiated fenugreek and albendazole-treated, and infected blue LED irradiated fenugreek and albendazole-treated. Mice were sacrificed on the 7th-day post-infection for the intestinal phase and the 40th day for the muscular phase. Small intestine, muscle tissues, and serum samples were collected to assess parasitic load, histopathological changes, TGF-β immunohistochemical expression, serum IFN-γ levels, oxidative stress markers (MDA, nitrate/SOD, and catalase), and BAX gene expression as an apoptotic marker. Metabolomic profiling of extract was pursued to spot differential expression of metabolites. Results: Study outcomes demonstrated that blue LED irradiated and non-irradiated fenugreek seed extracts combined with albendazole exhibited superior efficacy in reducing adult and larval burdens, improving pathological changes, decreasing IFN-γ levels, mitigating oxidative stress (reduced MDA and nitrate along with elevated SOD and catalase), and downregulating BAX expression. The observed metabolic differences were primarily driven by the upregulation of steroids, downregulation of most alkaloids, and dysregulation various flavonoids in the irradiated extract. Conclusion: Blue LED irradiated and non-irradiated fenugreek seed extracts can enhance albendazole's activity against trichinosis. © The Author(s) 2025.

Keywords

Albendazole; Apoptotic gene; BAX; Fenugreek; IFN-γ; Oxidative stress markers; TGF-β; Trichinosis; Animals; Female; Mice; Oxidative Stress; Plant Extracts; Seeds; Trichinellosis; Trigonella; plant extract; animal; chemistry; drug effect; drug therapy; mouse; parasitology; pathology; plant seed; radiation response

Citation Information

Scopus Citations: 1

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