Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Atta S.A.; Fahmy Z.H.; Selim E.A.H.; Aboushousha T.; Mostafa R.R.

Journal: BMC Infectious Diseases

Publisher: BioMed Central Ltd

Publication Date: 28 November 2024

Volume / Issue: Volume 24 / Issue 1

Article No.: 1360

ISSN: 14712334

DOI: 10.1186/s12879-024-10202-9

Scopus: View on Scopus

PubMed: 39609767

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Atta S.A., Department of Immunology, Theodor Bilharz Research Institute, Cairo, Egypt; Fahmy Z.H., Department of Parasitology, Theodor Bilharz Research Institute, Cairo, Egypt; Selim E.A.H., Department of Parasitology, Theodor Bilharz Research Institute, Cairo, Egypt; Aboushousha T., Department of Pathology, Theodor Bilharz Research Institute, Cairo, Egypt; Mostafa R.R., Departments of Medical Parasitology Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

Trichinellosis is a zoonotic, foodborne parasitic infection causing muscle damage. This study investigated the potential therapeutic effects of the commercially available probiotic treatment Linex, both alone and in combination with Albendazole (ALB), on the intestinal and muscular stages of Trichinella spiralis infection in mice, assessing outcomes through parasitological, immunological, and histopathological measures. This study is the first to demonstrate the synergistic effect of combining the commercially available probiotic Linex with Albendazole for trichinellosis treatment. By enhancing both parasitological and immunological outcomes, this combined therapy not only significantly reduces parasite burden but also modulates the immune response, shifting it toward a protective Th1 profile. In parasitological terms, the highest adult and larval count reduction was observed in combined Linex and Albendazole treatment (100%, 97.7%) respectively. Lesser percentage of reduction were recorded in Linex alone therapy (43.2%, 88.4%) respectively. Histopathologically there was amelioration of the inflammatory cellular infiltration in all treated groups with best results in combined Linex and Albendazole treatment. Immunologically, serum IFN-γ levels increased significantly in all treated groups with highest levels in combined Linex and Albendazole treatment, while IL-4 and IL-13 level decreased significantly in all treated groups with best results observed in Linex alone treatment. To conclude; combined Linex and Albendazole treatment of mice infected with T. spirals could ameliorate the infection and improve the immune response. © The Author(s) 2024.

Keywords

IFN-γ; IL-13; IL-4; Linex; Probiotic; Trichinellosis; Albendazole; Animals; Anthelmintics; Disease Models, Animal; Female; Interferon-gamma; Interleukin-4; Mice; Mice, Inbred BALB C; Probiotics; Trichinella spiralis; gamma interferon; interleukin 4; anthelmintic agent; Il4 protein, mouse; probiotic agent; analysis of variance; animal experiment; animal model; animal tissue; Article; cell infiltration; controlled study; enzyme linked immunosorbent assay; euthanasia; follitropin blood level; foodborne pathogen; histology; histopathology; immune response; mouse; muscle injury; nonhuman; paraffin embedding; parasite load; parasitosis; phagocytosis; sandwich ELISA; staining; statistical analysis; Trichinella; trichinosis; visual acuity; zoonotic transmission; animal; Bagg albino mouse; blood; disease model; drug therapy; immunology; parasitology

Citation Information

Scopus Citations: 5

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