Therapeutic potential of platelet rich plasma against experimental Cryptosporidium parvum infection: in vivo study in immunosuppressed mice

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Mahmoud N.R.; Younis A.I.; Zalat R.S.; Soliman A.S.A.; Khater M.M.

Journal: Journal of Parasitic Diseases

Publisher: Springer

Publication Date: 7 August 2024

Volume / Issue: Volume 48 / Issue 4

Pages: 849–859

ISSN: 9717196

DOI: 10.1007/s12639-024-01713-y

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Green Open Access

Authors and Affiliations

Mahmoud N.R., Medical Parasitology Department, Kasr Al-Ainy, Faculty of Medicine, Cairo University, Giza, Egypt; Younis A.I., Medical Parasitology Department, Kasr Al-Ainy, Faculty of Medicine, Cairo University, Giza, Egypt; Zalat R.S., Parasitology Department, Theodor Bilharz Research Institute, Giza, Imbaba, Egypt; Soliman A.S.A., Pathology Department, National Research Centre, Giza, Egypt; Khater M.M., Medical Parasitology Department, Kasr Al-Ainy, Faculty of Medicine, Cairo University, Giza, Egypt

Abstract

The present study was designed to assess the possible effects of platelet rich plasma (PRP) when used individually and in combination with nitazoxanide (NTZ) on experimental Cryptosporidium parvum (C. parvum) infection. It was conducted on 100 male albino mice, laboratory bred in Theodore Bilharz Research Institute. Starting from the 7th day post infection (p.i), therapeutics were given to immunosuppressed infected mice, which were divided as follows; oral NTZ treated group (0.2 mg/g/day for 6 consecutive days), six-PRP-treated groups (0.5 μl/g/week) to be administered intravenously (IV) in 1st, 2nd, 3rd week as PRP alone in (3 groups) and combined with oral NTZ (0.2 mg/g/day for 6 consecutive days) in (3 groups). Parasitological, histopathological and immunohistochemical assessments of therapeutics under study were done. Fecal pellets collected from groups at different intervals were stained using modified Ziehl–Neelsen and examined under microscope. Among PRP-treated groups, the highest significant percentage of oocyst reduction (89.96%) was observed in the group received 3 doses of PRP in combination with NTZ on the 35th day post infection. Likewise, the histopathological examination of small intestinal tissue sections showed improvement in villous architecture with mild to moderate stunting and moderate inflammatory infiltrates in lamina propria. Immunohistochemical staining of small intestinal tissue sections showed moderate increase in the expression of TGF-β1. Therefore, PRP can be a novel strategy in the treatment of cryptosporidiosis particularly when combined with NTZ. © Indian Society for Parasitology 2024.

Keywords

Cryptosporidium parvum; Murine infection; Nitazoxanide; Platelet rich plasma; Transforming growth factor-β1; dexamethasone; transforming growth factor beta1; abscess; animal experiment; animal model; animal tissue; Article; cell infiltration; controlled study; cryptosporidiosis; hair loss; hematological parameters; histopathology; immunohistochemistry; immunosuppressive treatment; in vivo study; infection control; intestine tissue; lamina propria; male; mouse; nonhuman; oocyst; plasma cell; polymerase chain reaction; skin ulcer; sporozoite; stunting; thrombocyte rich plasma

Citation Information

Scopus Citations: 0

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