Comparative study between three carbonaceous nanoblades and nanodarts for antimicrobial applications

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Selim M.S.; Azzam A.M.; Shenashen M.A.; Higazy S.A.; Mostafa B.B.; El-Safty S.A.

Journal: Journal of Environmental Sciences (China)

Publisher: Chinese Academy of Sciences

Publication Date: February 2024

Volume / Issue: Volume 136

Pages: 594–605

ISSN: 10010742

DOI: 10.1016/j.jes.2023.02.036

Scopus: View on Scopus

PubMed: 37923468

Document Type: Article

Access: All Open Access; Green Open Access

Authors and Affiliations

Selim M.S., National Institute for Materials Science (NIMS), 1-2-1 Sengen, Ibaraki-ken, Tsukuba-Shi, 305-0047, Japan, Petroleum Application Department, Egyptian Petroleum Research Institute, Nasr City, 11727, Egypt; Azzam A.M., National Institute for Materials Science (NIMS), 1-2-1 Sengen, Ibaraki-ken, Tsukuba-Shi, 305-0047, Japan, Department of Environmental Research, Theodor Bilharz Research Institute, Giza, Egypt; Shenashen M.A., National Institute for Materials Science (NIMS), 1-2-1 Sengen, Ibaraki-ken, Tsukuba-Shi, 305-0047, Japan, Petroleum Application Department, Egyptian Petroleum Research Institute, Nasr City, 11727, Egypt; Higazy S.A., Petroleum Application Department, Egyptian Petroleum Research Institute, Nasr City, 11727, Egypt; Mostafa B.B., Department of Environmental Research, Theodor Bilharz Research Institute, Giza, Egypt; El-Safty S.A., National Institute for Materials Science (NIMS), 1-2-1 Sengen, Ibaraki-ken, Tsukuba-Shi, 305-0047, Japan

Abstract

The design of nanostructured materials occupies a privileged position in the development and management of affordable and effective technology in the antibacterial sector. Here, we discuss the antimicrobial properties of three carbonaceous nanoblades and nanodarts materials of graphene oxide (GO), reduced graphene oxide (RGO), and single-wall carbon nanotubes (SWCNTs) that have a mechano-bactericidal effect, and the ability to piercing or slicing bacterial membranes. To demonstrate the significance of size, morphology and composition on the antibacterial activity mechanism, the designed nanomaterials have been characterized. The minimum inhibitory concentration (MIC), standard agar well diffusion, and transmission electron microscopy were utilized to evaluate the antibacterial activity of GO, RGO, and SWCNTs. Based on the evidence obtained, the three carbonaceous materials exhibit activity against all microbial strains tested by completely encapsulating bacterial cells and causing morphological disruption by degrading the microbial cell membrane in the order of RGO > GO > SWCNTs. Because of the external cell wall structure and outer membrane proteins, the synthesized carbonaceous nanomaterials exhibited higher antibacterial activity against Gram-positive bacterial strains than Gram-negative and fungal microorganisms. RGO had the lowest MIC values (0.062, 0.125, and 0.25 mg/mL against B. subtilis, S. aureus, and E. coli, respectively), as well as minimum fungal concentrations (0.5 mg/mL for both A. fumigatus and C. albicans). At 12 hr, the cell viability values against tested microbial strains were completely suppressed. Cell lysis and death occurred as a result of severe membrane damage caused by microorganisms perched on RGO nanoblades. Our work gives an insight into the design of effective graphene-based antimicrobial materials for water treatment and remediation. © 2023

Keywords

Antimicrobial properties; Cell viability; Gram-positive bacteria; Mechano-bactericidal effect; Nanoblades; RGO nanosheets; Anti-Bacterial Agents; Escherichia coli; Graphite; Microbial Sensitivity Tests; Nanotubes, Carbon; Staphylococcus aureus; Bactericides; Biosynthesis; Cells; Cytology; Graphene; Microwave integrated circuits; Proteins; Single-walled carbon nanotubes (SWCN); Water treatment; antiinfective agent; carbon nanotube; graphene oxide; Anti-microbial properties; Bactericidal effects; Gram-positive bacterium; Graphene oxide nanosheet; Graphene oxides; Reduced graphene oxide nanosheet; Reduced graphene oxides; antimicrobial activity; cell organelle; comparative study; concentration (composition); experimental study; nanoparticle; nanotechnology; chemistry; microbial sensitivity test; High resolution transmission electron microscopy

Citation Information

Scopus Citations: 28

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