Is bladder outlet obstruction rat model to induce overactive bladder (OAB) has similarity to human OAB? Research on the events in smooth muscle, collagen, interstitial cell and telocyte distribution

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Wishahi M.; Hassan S.; Kamal N.; Badawy M.; Hafiz E.

Journal: BMC Research Notes

Publisher: BioMed Central Ltd

Publication Date: 11 January 2024

Volume / Issue: Volume 17 / Issue 1

Article No.: 22

ISSN: 17560500

DOI: 10.1186/s13104-023-06681-9

Scopus: View on Scopus

PubMed: 38212840

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Wishahi M., Department of Urology, Theodor Bilharz Research Institute, P.O. 30, Warrak El-Hadar, Cairo, Imbaba, Giza, 12411, Egypt; Hassan S., Department of Pathology and Electron microscopy, Theodor Bilharz Research Institute, Cairo, Egypt; Kamal N., Department of Anesthesia, Theodor Bilharz Research Institute, Cairo, Egypt; Badawy M., Department of Urology, Theodor Bilharz Research Institute, P.O. 30, Warrak El-Hadar, Cairo, Imbaba, Giza, 12411, Egypt; Hafiz E., Department of Pathology and Electron microscopy, Theodor Bilharz Research Institute, Cairo, Egypt

Abstract

Background: Cellular and cytoskeletal events of overactive bladder (OAB) have not been sufficiently explored in human bladder due to different limitations. Bladder outlet obstruction (BOO) had been induced in different animal models with different methods to induce (OAB). Similarity of the animal models of BOO to the human OAB is postulated but has not been confirmed. The interstitial cells of Cajal (ICCs), and telocytes (TCs) are an important players in smooth muscles conductivity, they had not been well investigated in the previous BOO models. Objectives are to investigate the morphological pattern of cellular, cytoskeleton and telocytes distribution in BOO rat model and to match the events in two time periods and compare it to the findings in real-world human OAB. Methods: Female Sprague-Dawley rats (Rattus norvegicus) were randomly divided into: sham (n = 10), BOO 6 W (n = 10), BOO 8 W (n = 10). Operative procedure to Induce BOO was done under anesthesia with intraperitoneal Ketamine administration. The Effect of induction of BOO was evaluated after 6 and 8 weeks. The rats were anesthetized, and the urinary bladder was removed, while the rat was unconscious under anaesthesia it was transferred to the inhalation anaesthesia cage for euthanasia, rats were sacrificed under light anesthesia using isoflurane. Care of animals, surgical procedure, and euthanasia adhered to Guide for the Care and Use of Laboratory Animals, and AVMA Guidelines for the Euthanasia of Animals. The retrieved bladder was processed for examination with histopathology, immunohistochemistry (IHC), and transmission electron microscopy (EM). Results: Histological examination of the bladder shows thinner urothelium, condensation of collagen between muscle bundles. IHC with c-kit shows the excess distribution of ICCs between smooth muscle bundles. EM shows frequent distribution of TCs that were situated between collagen fibers. Finings in BOO 6 W group and BOO 8 W group were comparable. Conclusion: The animal model study demonstrated increased collagen/ smooth muscle ratio, high intensity of ICCs and presence of TCs. Findings show that a minimally invasive procedure to induce BOO in rats had resulted in an OAB that has morphological changes that were stable in 6 & 8 weeks. We demonstrated the distribution of TCs and ICCs in the rat animal model and defined them. The population of TCs in the BOO rat model is described for the first time, suggests that the TCs and ICCs may contribute to the pathophysiology of OAB. Similarity of animal model to human events OAB was demonstrated. These findings warrant further study to define the role of TCs in OAB. Clinical trial registry: The study does not require a clinical trial registration; it is an experimental animal study in basic science and does not include human subjects. © 2024, The Author(s).

Keywords

Bladder outlet obstruction; Experimental animal; Intersticial cells; Overactive bladder; Rats; Smooth muscles; Telocytes; Animals; Collagen; Disease Models, Animal; Female; Humans; Interstitial Cells of Cajal; Muscle, Smooth; Rats, Sprague-Dawley; Urinary Bladder Neck Obstruction; Urinary Bladder, Overactive; animal; bladder obstruction; controlled study; disease model; human; interstitial cell of Cajal; randomized controlled trial; rat; smooth muscle; Sprague Dawley rat; telocyte

Citation Information

Scopus Citations: 6

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