Cytotoxicity, Antioxidant Activities, GC-MS and HPLC Fingerprint Analyses of Different Extracts of Desmodium tortuosum (Sw.) DC

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Elshazly M.; Hamada F.A.; Refahy L.A.

Journal: Jordan Journal of Pharmaceutical Sciences

Publisher: University of Jordan,Deanship of Scientific Research

Publication Date: 25 December 2023

Volume / Issue: Volume 16 / Issue 4

Pages: 690–713

ISSN: 19957157

DOI: 10.35516/jjps.v16i4.930

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Elshazly M., Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Egypt; Hamada F.A., Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Egypt; Refahy L.A., Botany department, Faculty of science, Aswan University, Egypt

Abstract

The family Fabaceae is the third-largest flowering plant family, and the genus Desmodium has exhibited a wide range of biological activities and a variety of chemical constituents. In the present study, different extracts of Desmodium tortuosum were evaluated for their cytotoxic and antioxidant activities, as well as their total phenolic content (TPC). The antioxidant activities were estimated using the 1,1'-diphenyl-2-picraylhydrazyl free radical (DPPH), while the cytotoxic activity was evaluated via the brine shrimp lethality test (BSLT). The antioxidant activity results revealed that the DPPH radical scavenging activity (SC50) ranged from 1.12 to 61.22 µg/ml with respect to ascorbic acid (SC50 = 7.45 µg/ml). Among all tested fractions, 90% methanol was the most active. On the other hand, the cytotoxic activities were arranged as follows: n-BuOH (LC50 = 310), EtOAc (LC50 = 350), and 70% methanol (LC50 = 380). High-Performance Liquid Chromatography-Fingerprint analyses were used to determine the chemical composition and relative proportions of phenolic compounds. GC-MS analysis indicated the presence of fatty acids and other compounds. The major identified compounds were Benzene (1-butyloctyl) (11.88%) and Himachalene <α-> (11.08%) for the ethyl acetate extract and 10-Undecenoic acid, methyl ester (25.50%) for unsaponifiable matter. © 2023 DSR Publishers/The University of Jordan.

Keywords

Antioxidant; Cytotoxicity; Desmodium tortuosum; GC-MS; HPLC; 10 undecenoic acid; ascorbic acid; benzene; Desmodium tortuosum extract; methanol; plant extract; unclassified drug; antioxidant activity; Article; biological activity; brine shrimp lethality test; chemical composition; column chromatography; controlled study; DPPH radical scavenging assay; Fabaceae; high performance liquid chromatography; LC50; lethality; mass fragmentography; nonhuman; oxidative stress

Citation Information

Scopus Citations: 2

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