Carbapenemase producing Enterobacterales clinical isolates from a tertiary care hospital in Egypt

Theodor Bilharz Research Institute

Bibliographic Information

Authors: El Defrawy I.; Salem D.; Ali G.; Gamal D.; El Dabaa E.; Diab M.; Alyan S.A.-E.; Sallam M.K.

Journal: Beni-Suef University Journal of Basic and Applied Sciences

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: 3 November 2023

Volume / Issue: Volume 12 / Issue 1

Article No.: 98

ISSN: 23148535

DOI: 10.1186/s43088-023-00437-x

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

El Defrawy I., Microbiology Department, Theodor Bilharz Research Institute (TBRI), 30 Imbaba, P.O box 12411, Giza, Egypt; Salem D., Microbiology Department, Theodor Bilharz Research Institute (TBRI), 30 Imbaba, P.O box 12411, Giza, Egypt; Ali G., Microbiology Department, Theodor Bilharz Research Institute (TBRI), 30 Imbaba, P.O box 12411, Giza, Egypt; Gamal D., Microbiology Department, Theodor Bilharz Research Institute (TBRI), 30 Imbaba, P.O box 12411, Giza, Egypt; El Dabaa E., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), 30 Imbaba, P.O box 12411, Giza, Egypt; Diab M., Microbiology Department, Theodor Bilharz Research Institute (TBRI), 30 Imbaba, P.O box 12411, Giza, Egypt; Alyan S.A.-E., Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Sallam M.K., Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

Background: Carbapenem resistant Enterobacterales (CRE) is on the rise globally, triggering a significant health threat and a substantial concern for infection control management. We aimed to detect and characterize carbapenemases producing Enterobacterales (CPE) clinical isolates over a period of nearly one-year duration in Theodor Bilharz Research Institute, a tertiary care hospital in Egypt through molecular and phenotypic methods using carbapenemase detection combination inhibitor disk set (Enterobacterales) MASTDISCS ID (MDI) (MAST, UK), with the addition of temocillin disk. Results: CRE represented 6.5% of Enterobacterales. Healthcare-associated infections were frequently high representing 87% of the CRE isolated from hospitalized patients. Most of the CRE isolates were Klebsiella pneumonia (68%) followed by Escherichia coli (22%), Enterobacter cloacae (4%), Serratia marcescens (4%) and Citrobacter freundii (2%). Phenotypic detection revealed metallo-β lactamases in 84% of isolates, followed by oxacillinase-48 {(OXA-48) 6%} and Klebsiella pneumoniae carbapenemase in 2% of the isolates. The most prevalent gene detected by conventional PCR was bla NDM (84%) followed by bla OXA-48 (6%) and bla KPC (2%). Excellent agreement was found between PCR and MDI for detection of carbapenemase production. Conclusions: NDM carbapenemase is prevalent in our hospital. Carbapenemase detection combination inhibitor disk set (Enterobacterales) MASTDISCS ID is a useful tool for rapid and precise confirmation of the detection of CPE. © 2023, The Author(s).

Keywords

CRE; Infection control; KPC; MASTDISCS ID; MBLs; NDM; OXA-48

Citation Information

Scopus Citations: 11

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