Garden Cress Seed Oil Abrogates Testicular Oxidative Injury and NF-kB-Mediated Inflammation in Diabetic Mice

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abu-Khudir R.; Badr G.M.; Abd El-Moaty H.I.; Hamad R.S.; Al Abdulsalam N.K.; Abdelrahem A.S.A.; Alqarni S.; Alkuwayti M.A.; Salam S.A.; Abd El-Kareem H.F.

Journal: International Journal of Molecular Sciences

Publisher: Multidisciplinary Digital Publishing Institute (MDPI)

Publication Date: 23 October 2023

Volume / Issue: Volume 24 / Issue 20

Article No.: 15478

ISSN: 16616596

DOI: 10.3390/ijms242015478

Scopus: View on Scopus

PubMed: 37895159

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Abu-Khudir R., Department of Chemistry, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa, 31982, Saudi Arabia, Department of Chemistry, Biochemistry Division, Faculty of Science, Tanta University, Tanta, 31527, Egypt; Badr G.M., Department of Zoology, Faculty of Science, Ain Shams University, Cairo, 11566, Egypt; Abd El-Moaty H.I., Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa, 31982, Saudi Arabia, Medicinal and Aromatic Plants Department, Desert Research Center El-Mataria, Cairo, 11753, Egypt; Hamad R.S., Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa, 31982, Saudi Arabia, Central Laboratory, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Al Abdulsalam N.K., Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa, 31982, Saudi Arabia; Abdelrahem A.S.A., Department of Nursing, College of Applied Medical Science, King Faisal University, P.O. Box 380, Al-Ahsa, 31982, Saudi Arabia; Alqarni S., Department of Clinical Nutrition, College of Applied Medical Science King Faisal University, P.O. Box 380, Al-Ahsa, 31982, Saudi Arabia; Alkuwayti M.A., Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa, 31982, Saudi Arabia; Salam S.A., Department of Zoology, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt; Abd El-Kareem H.F., Department of Zoology, Faculty of Science, Ain Shams University, Cairo, 11566, Egypt

Abstract

Diabetes mellitus is a metabolic disorder associated with various complications encompassing male reproductive dysfunction. The present study aimed to investigate the therapeutic potential of biologically active Lepidium sativum seed oil (LSO) against the testicular dysfunction associated with streptozotocin (STZ)-induced diabetes. Male adults (n = 24) were divided into four groups: control, LSO-administered, diabetic (D), and LSO-treated diabetic (D+LSO) groups. LSO was extracted from L. sativum seeds, and its chemical composition was determined using GC-MS. Serum testosterone levels, testicular enzymatic antioxidants (catalase (CAT) and superoxide dismutase (SOD)), an oxidative stress (OS) biomarker, malondialdehyde (MDA), pro-inflammatory markers (NF-kB, IL-1, IL-6, and TNF-α), and the expression level of NF-kB were assessed. In addition, histopathological changes were evaluated in testicular tissues. The results obtained showed that the chemical composition of LSO indicated its enrichment mainly with γ-tocopherol (62.1%), followed by 2-methylhexacosane (8.12%), butylated hydroxytoluene (8.04%), 10-Methylnonadecane (4.81%), and δ-tocopherol (3.91%). Moreover, LSO administration in the D+LSO mice significantly increased testosterone levels and ameliorated the observed testicular oxidative damage, inflammatory response, and reduced NF-kB expression compared to the diabetic mice. Biochemical and molecular analyses confirmed the histological results. In conclusion, LSO may prevent the progression of diabetes-induced impairment in the testes through inhibition of the OS- and NF-kB-mediated inflammatory response. © 2023 by the authors.

Keywords

diabetes mellitus; histopathology; inflammation; Lepidium sativum L. seed oil; oxidative stress; testis; Animals; Antioxidants; Diabetes Mellitus, Experimental; Humans; Lepidium sativum; Male; Mice; NF-kappa B; Plant Oils; Testicular Diseases; Testosterone; 1 bromotetracosane; 1 iodotridecane; 10 methylnonadecane; 2 methylhexacosane; 2 methyloctacosane; 2 methyltetracosane; 6 pentadecen 1 ol acetate; biological marker; butylcresol; catalase; delta tocopherol; eosin; gamma tocopherol; hematoxylin; immunoglobulin enhancer binding protein; interleukin 1; interleukin 6; malonaldehyde; phenol derivative; streptozocin; superoxide dismutase; tumor necrosis factor; unclassified drug; vegetable oil; antioxidant; adult; animal experiment; animal model; animal tissue; Article; chemical composition; controlled study; diabetic complication; gene expression level; interstitium; mass fragmentography; mouse; NF kB signaling; nonhuman; retention time; seminiferous tubule; streptozotocin-induced diabetes mellitus; testis injury; testis tissue; testosterone blood level; Western blotting; animal; experimental diabetes mellitus; human; metabolism; testis disease

Citation Information

Scopus Citations: 15

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