Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation

Theodor Bilharz Research Institute

Bibliographic Information

Authors: El-Sayed H.; Morad M.Y.; Sonbol H.; Hammam O.A.; Abd El-Hameed R.M.; Ellethy R.A.; Ibrahim A.M.; Hamada M.A.

Journal: Microorganisms

Publisher: Multidisciplinary Digital Publishing Institute (MDPI)

Publication Date: 19 September 2023

Volume / Issue: Volume 11 / Issue 9

Article No.: 2341

ISSN: 20762607

DOI: 10.3390/microorganisms11092341

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

El-Sayed H., Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan, 11795, Egypt; Morad M.Y., Zoology and Entomology Department, Faculty of Science, Helwan University, Helwan, 11795, Egypt; Sonbol H., Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia; Hammam O.A., Pathology Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Abd El-Hameed R.M., Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan, 11795, Egypt; Ellethy R.A., Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, 11795, Egypt; Ibrahim A.M., Medical Malacology Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Hamada M.A., Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan, 11795, Egypt

Abstract

Bacterial-associated wound infections are an obstacle for individuals and the medical industry. Developing versatile, antibiotic-free therapies helps heal wounds more quickly and efficiently. In the current study, fungal metabolites were employed as a reducing agent in fabricating selenium nanoparticles (SeNPs) for improved antibacterial and wound healing properties. Utilizing UV-visible spectroscopy, dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), and electron microscopic examination, the properties of the synthesized nanoparticles were extensively evaluated. Myco-synthesized SeNPs demonstrated strong antibacterial activity against Staphylococcus aureus ATCC 6538 with a minimum inhibitory concentration of 0.3125 mg/mL, reducing cell number and shape distortion in scanning electron microscope (SEM) images. SeNPs’ topical administration significantly reduced wound area and healing time, exhibiting the least bacterial load after six days compared to controls. After six and 11 days of treatment, SeNPs could decrease proinflammatory cytokines IL-6 and TNF-α production. The histopathological investigation showed a healed ulcer with moderate infiltration of inflammatory cells after exposing mice’s skin to SeNPs for six and 11 days. The docking interaction indicated that SeNPs were highly efficient against the IL-6 and TNF-α binding receptors. These findings imply that myco-fabricated SeNPs might be used as topically applied antimicrobial agents for treating skin infections and wounds. © 2023 by the authors.

Keywords

antibacterial; docking study; pro-inflammatory cytokines; selenium nanoparticles; Staphylococcus aureus; wound healing

Citation Information

Scopus Citations: 29

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