The Molluscicidal Activity of Green Synthesized Copper Oxide–Based Annona squamosa Seed Extract Nanoparticles on the Feeding Behavior, Biochemical, Molecular, and Immunohistochemical Alterations of Biomphalaria alexandrina Snails

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Hussein A.A.A.; Abd El-latif M.B.; Saad El-Din M.I.; El-Shenawy N.S.; Hammam O.; Ibrahim A.M.

Journal: Biological Trace Element Research

Publisher: Springer

Publication Date: 30 August 2023

Volume / Issue: Volume 202 / Issue 5

Pages: 2327–2337

ISSN: 1634984

DOI: 10.1007/s12011-023-03823-9

Scopus: View on Scopus

PubMed: 37648936

Document Type: Article

Access: All Open Access; Hybrid Gold Open Access

Authors and Affiliations

Hussein A.A.A., Medical Malacology Department, Theodor Bilharz Research Institute, Giza, Egypt; Abd El-latif M.B., Environmental Research Department, Theodor Bilharz Research Institute, Giza, Egypt; Saad El-Din M.I., Zoology Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt; El-Shenawy N.S., Zoology Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt; Hammam O., Pathology Department, Theodore Bilharz Research Institute, Giza, Egypt; Ibrahim A.M., Medical Malacology Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Because of their low ecological impact, plant molluscicides have garnered much attention. The work aimed to find out if Annona squamosa (AS) seed extract has a molluscicidal impact on Biomphalaria alexandrina snails and enhances this extract by adding CuO nanoparticles (NPs). Using a scanning electron microscope (SEM), transmission electron microscope (TEM), and PANalytical X’Pert PRO X-ray diffractometer (XRD), the presence of the green A. squamosa-based CuO NPs (AS-CuO NPs) was confirmed. After 24 h of exposure, the half-lethal concentration (LC50) of AS-CuO NPs was more toxic to mature B. alexandrina than the aqueous extract of AS seeds (LC50: 119.25 mg/L vs. 169.03 mg/L). The results show that snails exposed to sublethal doses of AS-CuO NPs at LC10 or LC25 (95.4 or 106.7 mg/L, respectively) had much higher glucose levels and alkaline phosphatase activity than those not exposed. Nevertheless, there was no discernible change in the protein content in general or glycogen phosphorylase production. Histological and immunohistochemical analysis showed that snails exposed to A. squamosa-derived CuO NPs LC10 had shrinking digestive tubules and degeneration as well as vacuolation of many digestive, secretory, ova, and sperm cells, with PCNA expressing positively in the hermaphrodite gland and digestive tubule cells. The toxic profile of green CuO NPs produced by A. squamosa may damage the biological activity of B. alexandrina snails; thus, this compound could be used as a molluscicidal base. Furthermore, B. alexandrina proved to be a useful biomarker of nanomaterial contamination. © The Author(s) 2023.

Keywords

Annona squamosa L; Biomphalaria alexandrina; Characterization of CuO NPs; Copper oxide nanoparticles; Molluscicidal activity; Animals; Annona; Biomphalaria; Copper; Feeding Behavior; Molluscacides; Nanoparticles; Oxides; Plant Extracts; Seeds; alkaline phosphatase; copper oxide nanoparticle; glucose; glycogen phosphorylase; cuprous oxide; molluscacide; nanoparticle; oxide; plant extract; Annona squamosa; aqueous solution; Article; biochemical analysis; cell vacuole; controlled study; enzyme activity; green synthesis; histology; immunohistochemistry; LC50; nonhuman; plant seed; protein content; scanning electron microscopy; synthesis; transmission electron microscopy; X ray diffraction; animal

Citation Information

Scopus Citations: 15

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