Biological activity, chemical profiling and molecular docking of tissue extracts of the sea snail Trochus erithreus

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Zayed K.M.; Ghareeb M.A.; Habib M.R.; El-Einin H.M.A.; Ali R.E.M.; El-Karim R.M.G.; Sabour R.; Hamed A.A.

Journal: Journal of Applied Pharmaceutical Science

Publisher: Open Science Publishers LLP Inc.

Publication Date: 1 June 2023

Volume / Issue: Volume 13 / Issue 6

Pages: 199–210

ISSN: 22313354

DOI: 10.7324/JAPS.2023.119765

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Zayed K.M., Medical Malacology Department, Theodor Bilharz Research Institute, Corniche El-Nile, Warrak El-Hadar,Imbaba (P.O. 30), Giza, 12411, Egypt; Ghareeb M.A., Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Corniche El-Nile, Warrak El-Hadar,Imbaba (P.O. 30), Giza, 12411, Egypt; Habib M.R., Medical Malacology Department, Theodor Bilharz Research Institute, Corniche El-Nile, Warrak El-Hadar,Imbaba (P.O. 30), Giza, 12411, Egypt; El-Einin H.M.A., Medical Malacology Department, Theodor Bilharz Research Institute, Corniche El-Nile, Warrak El-Hadar,Imbaba (P.O. 30), Giza, 12411, Egypt; Ali R.E.M., Medical Malacology Department, Theodor Bilharz Research Institute, Corniche El-Nile, Warrak El-Hadar,Imbaba (P.O. 30), Giza, 12411, Egypt; El-Karim R.M.G., Medical Malacology Department, Theodor Bilharz Research Institute, Corniche El-Nile, Warrak El-Hadar,Imbaba (P.O. 30), Giza, 12411, Egypt; Sabour R., Medicinal Pharmaceutical Chemistry and Drug Design Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt; Hamed A.A., Microbial Chemistry Department, National Research Centre, 33 El-Buhouth St.,Dokki, Giza, 12622, Egypt

Abstract

The biodiversity of marine mollusks, along with their worldwide availability and ease of harvesting, makes them an enticing subject for scientific research. Mollusks are frequently the target of pharmaceutical scientists who are searching for novel compounds to benefit the population. This study targets the investigation of the antioxidant, antibiofilm, antimicrobial, and cytotoxic activities of different solvent extracts of marine mollusk Trochus erithreus. The chemical constituents of the most promising extract and molecular docking analyses are also investigated. Results showed that ethyl alcohol extract had the highest phenolic content (213.90 ± 4.88 mg GAE/ g extract), total antioxidant activity (545.33 ± 4.02 mg AAE/ g extract), and antibacterial activity against Staphylococcus aureus (31.32 ± 1.49), while the acetone extract had the highest scavenging effect against 2,2-diphenyl-1-picryl-hydrazyl radical (IC50: 53.59 ± 1.71 μg/ml). The acetone extract also displayed pronounced antibacterial activity against Proteus vulgaris with an inhibition ratio of up to 67.93% and it had potent cytotoxic effects against both prostate (IC50: 1.666 ± 0.07 μg/ml) and human lung carcinoma cell lines (IC50: 12.04 ± 0.55 μg/ml). Gas chromatography-mass spectrometry investigation of the acetone extract resulted in the recognition of 45 compounds. Bisabolol oxide A (24.63%), (E)-α-farnesene (7.89%), and tricyclo[8.2.0.0(2,5)] dodeca-3,6,8,11-tetraene (5.41%) were detected as major components. The molecular docking study demonstrated strong binding scores and modes of interactions of several major compounds, inside the active binding sites of bacterial enzymes, thymidylate kinase, DNA gyrase B, and DNA topoisomerase IV/subunit Bs. © 2023 Khaled M. Zayed et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License

Keywords

antibiofilm; antimicrobial; bioactivities; chemical constituents; Marine mollusca; molecular docking; DNA topoisomerase; DNA topoisomerase (ATP hydrolysing); DNA topoisomerase (ATP hydrolysing) B; DNA topoisomerase IV; tissue extract; animal experiment; animal tissue; antibacterial activity; antibiofilm activity; antifungal activity; antimicrobial activity; antioxidant activity; Article; biodiversity; biological activity; chemical fingerprinting; controlled study; cytotoxicity; DPPH radical scavenging assay; Enterococcus faecalis; Escherichia coli; HeLa cell line; human; human cell; IC50; Klebsiella; mass fragmentography; mollusc; MTT assay; nonhuman; Proteus vulgaris; Pseudomonas aeruginosa; snail; Staphylococcus aureus

Citation Information

Scopus Citations: 6

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