Profiling the volatile composition and cytotoxic assessment of the encapsulated Spirulina Platensis using alginate chitosan nanoparticles

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Fadl Allah W.M.; Shemis M.A.; Abd El Azeem E.M.; Osman E.E.A.; Mamdouh S.

Journal: Egyptian Journal of Chemistry

Publisher: National Information and Documentation Centre

Publication Date: 21 May 2023

Volume / Issue: Volume 66 / Issue 12

Pages: 511–527

ISSN: 4492285

DOI: 10.21608/ejchem.2023.199387.7718

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Fadl Allah W.M., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornaish El-Nile St., Giza, 12411, Egypt; Shemis M.A., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornaish El-Nile St., Giza, 12411, Egypt; Abd El Azeem E.M., Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile St., Giza, 12411, Egypt; Osman E.E.A., Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt; Mamdouh S., Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornaish El-Nile St., Giza, 12411, Egypt

Abstract

Spirulina platensis (S. platensis) is a blue-green microalga that belongs to the cyanobacteria family. The goal of this research was to investigate the anticancer activity of encapsulation S. platensis algae with Chitosan-Alginate polyelectrolyte nanoparticles on HepG-2, Caco-2, and Vero cell lines and profile its chemical components. S.platensis was extracted using 90% MeOH as a crude extract which was fractionated using different organic solvents. While Nanoencapsulation of 90% MeOH ext. and pet-ether fr. by Chitosan-Alginate polyelectrolyte nanoparticles were prepared by modified ionic gelation method. The chemical composition of 90% MeOH ext. was identified using the GC-MS technique. The 90% MeOH ext. of S. platensis showed high toxicity on HepG-2 and Caco-2 cells, more than the pet-ether fraction. While they were safe on Vero normal cells. Furthermore, nanoencapsulation of the 90%MeOH ext. and pet-ether fr. increased the cytotoxicity against HepG-2 and Caco-2 cells. Moreover, The effect of 90%MeOH ext. and pet-ether fr. NPs against HepG-2 and Caco-2 resulted in a significant increase in the relative expression of the Caspase-3 enzyme and a decrease in the expression of the TNFα gene and COX-2 enzyme more than the non-encapsulated form. The GC-MS analysis of the silylated 90 % MeOH ext. revealed the presence of 57 bioactive compounds including, alcohols, terpenes, organic acids, and others. ©2023 National Information and Documentation Center (NIDOC)

Keywords

Alginate; anti-cancer; GC-MS analysis; nanoencapsulation; Spirulina platensis

Citation Information

Scopus Citations: 5

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