Comprehensive chemical profiling of Bassia indica Wight. aerial parts extract using UPLC-ESI–MS/MS, and its antiparasitic activity in Trichinella spiralis infected mice: in silico supported in vivo study

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Mohammed M.M.D.; Heikal E.A.; Ellessy F.M.; Aboushousha T.; Ghareeb M.A.

Journal: BMC Complementary Medicine and Therapies

Publisher: BioMed Central Ltd

Publication Date: 18 May 2023

Volume / Issue: Volume 23 / Issue 1

Article No.: 161

ISSN: 26627671

DOI: 10.1186/s12906-023-03988-9

Scopus: View on Scopus

PubMed: 37202749

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Mohammed M.M.D., Pharmacognosy Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Dokki-12622, Egypt; Heikal E.A., Department of Medical Parasitology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt; Ellessy F.M., Department of Medical Parasitology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt; Aboushousha T., Department of Pathology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba, (P.O. 30), Giza, 12411, Egypt; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba, (P.O. 30), Giza, 12411, Egypt

Abstract

Background: Trichinellosis is a public health threat infected both animals and humans as a result of eating undercooked meat. It caused by Trichinella spiralis that has widespread drug resistance and even developed many sophisticated strategies for their survival, this increases the demand in searching for new anthelmintic drugs from natural source. Methods: Our objectives were to test the in vitro and in vivo anthelmintic activity of Bassia indica BuOH frac., and to characterize its chemical composition using UPLC-ESI–MS/MS. Besides an in silico molecular docking study with the prediction of the PreADMET properties. Results: In vitro investigation of B. indica BuOH frac., showed severe destruction of the adult worm and larvae, marked cuticle swelling, areas with vesicles, blebs and loss of annulations. This was assured via in vivo study, which revealed a significant reduction (P < 0.05) in the mean adult worm count with efficacy of 47.8% along with a significant decrease (P < 0.001) in the mean larval count per gram muscle with efficacy 80.7%. Histopathological examinations of the small intestine and muscular sections showed marked improvement. In addition, immunohistochemical findings demonstrated that B. indica BuOH frac. depressed the proinflammatory cytokines expressions of TNF-α, which was obviously upregulated by T. spiralis. Precise chemical investigation of the BuOH frac. using UPLC-ESI–MS/MS resulted in the identification of 13 oleanolic type triterpenoid saponins; oleanolic acid 3-O-6´-O-methyl-β-D-glucurono-pyranoside (1), chikusetsusaponin-IVa (2) and its methyl ester (3), chikusetsusaponin IV (4) and its methyl ester (5), momordin-Ic (6) and its methyl ester (7), betavulgaroside-I (8), -II (9) -IV (10), -X (11), licorice-saponin-C2 (12) and -J2 (13). In addition, 6 more phenolics were identified as syringaresinol (14), 3,4-di-O-caffeoylquinic acid (15), 3-O-caffeoyl-4-O-dihydrocaffeoylquinic acid (16), 3,4-di-O-caffeoylquinic acid butyl ester (17), 3,5-di-O-galloyl-4-O-digalloylquinic acid (18) and quercetin 3-O-(6´´-feruloyl)-sophoroside (19). The auspicious anthelmintic activity was further ascertained using in silico molecular docking approach that targeted certain protein receptors (β-tubulin monomer, tumor necrosis factor alpha (TNF-α), cysteine protease (Ts-CF1), calreticulin protein (Ts-CRT)), all the docked compounds (1–19) fit into the binding site of the active pocket with binding affinities noteworthy than albendazole. In addition, ADMET properties, drug score and drug likeness were predicted for all compounds. © 2023, The Author(s).

Keywords

Bassia indica; Chenopodiaceae; Molecular Docking; Phenolics; Saponins; Trichinella spiralis; Animals; Anthelmintics; Antiparasitic Agents; Humans; Mice; Molecular Docking Simulation; Plant Components, Aerial; Plant Extracts; Tandem Mass Spectrometry; Tumor Necrosis Factor-alpha; 3 O caffeoyl 4 O dihydro caffeoylquinic acid; 3,4 di O caffeoylquinic acid; 3,4 di O caffeoylquinic acid butyl ester; 3,5 di O galloyl 4 O digalloylquinic acid; Bassia indica extract; betavulgaroside; calreticulin; chikusetsu saponin; cysteine proteinase; licorice saponin; momordin; oleanolic acid; oleanolic acid 3 O 6 O methyl beta D glucurono pyranoside; plant extract; plant medicinal product; quercetin 3 O (6 feruloyl) sophoroside; triterpenoid; tumor necrosis factor; unclassified drug; 3,4-di-O-caffeoylquinic acid; anthelmintic agent; antiparasitic agent; amino acid sequence; animal experiment; animal model; animal tissue; anthelmintic activity; Article; Bassia; binding affinity; chemical composition; chemical fingerprinting; controlled study; crystal structure; drug absorption; drug distribution; drug excretion; drug metabolism; drug toxicity; electrospray mass spectrometry; histopathology; immunohistochemistry; larva; male; mouse; nonhuman; pharmacokinetic parameters; scanning electron microscopy; trichinosis; ultra performance liquid chromatography; upregulation; worm burden; aerial plant part; animal; human

Citation Information

Scopus Citations: 14

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