Nano magnetic-based ELISA and nano magnetic-based latex agglutination test for diagnosis of experimental trichinellosis

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Kamel H.H.; Elleboudy N.A.F.; Hasan A.N.; Ali I.R.; Mohammad O.S.

Journal: Journal of Parasitic Diseases

Publisher: Springer

Publication Date: 16 April 2023

Volume / Issue: Volume 47 / Issue 2

Pages: 400–409

ISSN: 9717196

DOI: 10.1007/s12639-023-01583-w

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Green Open Access

Authors and Affiliations

Kamel H.H., Medical Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Elleboudy N.A.F., Medical Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Hasan A.N., Medical Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Ali I.R., Parasitology Department, Theodore Bilharz Research Institute, Giza, Egypt; Mohammad O.S., Medical Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Abstract

Human trichinellosis is a worldwide foodborne public health threat. Detecting circulating antigens of Trichinella spiralis “T. spiralis” allows for an early diagnosis before larval encystation develops in skeletal muscles. For the first time, the present study aimed to formulate an effective nanomagnetic beads based-ELISA and -latex agglutination test (NMB-ELISA and NMB-LAT) to recognize T. spiralis adult worm crude extract antigen (AWCEA) in sera of experimentally infected mice. The study included thirty-eight mice classified into 3 groups; T. spiralis-infected group (GI) which was euthanized 6, 8, 10, 12, 14 days post-infection (dpi), other parasitic infections group (GII) and healthy control group (GIII). Rabbit anti-T. spiralis polyclonal antibodies (pAbs) were utilized to detect AWCEA in serum samples by sandwich ELISA, NMB-ELISA, and NMB-LAT. Using NMB-ELISA, AWCEA was detected in sera collected at 6 and 8 dpi, with a sensitivity of 50% and 75%, respectively, and a specificity of 100%. Whereas, sandwich ELISA and NMB-LAT couldn’t detect the antigen at the same time intervals. Both ELISA formats were able to detect the antigen in samples collected at 10, 12, and 14 dpi with a sensitivity of 100% for NMB-ELISA and 25%, 75%, and 100% respectively, for sandwich-ELISA. Yet, NMB-LAT couldn't detect AWCEA until 12 dpi with a sensitivity of 50% and specificity of 75%. In conclusion, NMB-ELISA is a promising sensitive tool for early and specific diagnosis of acute trichinellosis. The use of NMB-LAT could be a helpful screening procedure in field surveys. © 2023, Indian Society for Parasitology.

Keywords

Crude extract antigen; ELISA; Latex agglutination; Nanomagnetic beads; Trichinella spiralis; adult worm crude extract antigen; animal extract; horseradish peroxidase; immobilized antibody; pepsin A; polyclonal antibody; polystyrene; unclassified drug; adult; animal experiment; animal model; Article; conjugation; controlled study; cryptosporidiosis; diagnostic test accuracy study; enzyme linked immunosorbent assay; in vivo study; latex agglutination test; male; mouse; nanomagnetic based enzyme linked immunosorbent assay; nanomagnetic based latex agglutination test; nonhuman; parasite encystment; predictive value; quantitative analysis; sandwich ELISA; schistosomiasis mansoni; sensitivity and specificity; skeletal muscle; transmission electron microscopy; trichinosis

Citation Information

Scopus Citations: 3

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