In Vitro Cytotoxic Activity and Identification of Bioactive Compounds Isolated from Olea europaea and Syzygium aromaticum Plants

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Okasha H.; Abdel-Hady H.; Morsi E.A.; El-Wakil E.A.; Shemis M.A.

Journal: Pharmaceutical Chemistry Journal

Publisher: Springer

Publication Date: 18 November 2022

Volume / Issue: Volume 56 / Issue 8

Pages: 1123–1132

ISSN: 0091150X

DOI: 10.1007/s11094-022-02761-5

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Okasha H., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Giza, Egypt; Abdel-Hady H., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt; Morsi E.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt; El-Wakil E.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt; Shemis M.A., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Natural products have been conventional in traditional medicine and still used presently to cure many diseases. In the present study, extracts of two plants – Olea europaea and Syzygium aromaticum – were tested for their phytochemical compositions, cytotoxic effect on hepatocellular carcinoma cells, and chemical profile of the most active fractions. Both plants were extracted with 85% methanol and then fractionated with various solvents. Hemolysis and cytotoxicity effects on normal (Vero) and hepatocellular carcinoma (HepG2) cells were examined. Chemical profiles were studied using GC-MS and chromatographic isolation on silica gel. Phytochemical screening revealed that both plants contain carbohydrates, saponins and flavonoids, while steroids were found only in O. europaea and alkaloids were found only in S. aromaticum. Hemolysis assay showed complete safety of O. europaea, while S. aromaticum exhibited low toxicity. Furthermore, the EtOAc fraction of O. europaea and BuOH fraction of S. aromaticum showed the best IC50 against HepG2. The GC-MS analysis of EtOAc fraction of O. europaea revealed 15 compounds, the major one being α-linoleic acid methyl ester, and the chromatographic separation of BuOH fraction of S. aromaticum resulted in a pure compound of 3-O-methyl-butyrate-6,7,3',4'-tetrahydroxy-5-methoxy-8-methylflavan. Thus, the study proved that the two title plants have components possessing cytotoxic activity against HepG2 cells. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

chromatography; GC-MS; hemolysis; hepatocellular carcinoma; Olea europaea; Syzygium aromaticum; 11 eicosenoic acid methyl ester; 3 o methyl butyrate 6,7,3 4' tetrahydroxy 5 methoxy 8 methylflavan; alkaloid derivative; alpha linoleic acid methyl ester; analgesic agent; antiinflammatory agent; behenic acid methyl ester; butylcresol; carbohydrate; clove extract; cytotoxic agent; eicosanoic acid methyl ester; flavonoid; hemoglobin; heneicosanoic acid methyl ester; ibuprofen; lignoceric methyl ester; linoleic acid methyl ester; methyl margarate; methyl palmitolate; methyl stearate; myristic acid methyl ester; oleic acid methyl ester; olive extract; palmitic acid methyl ester; phytochemical; plant extract; saponin derivative; stearic acid methyl ester; steroid; tricosanoic acid methyl ester; unclassified drug; wound healing promoting agent; analgesic activity; animal cell; antiinflammatory activity; Article; cell viability; chemical composition; clove; comparative study; controlled study; cytotoxicity; drug identification; drug isolation; drug safety; drug screening; hemolysis assay; Hep-G2 cell line; hepatocellular carcinoma cell line; human; IC50; in vitro study; liver cell carcinoma; mass fragmentography; nonhuman; normal human; oligodendroglia; olive tree; plant leaf; proton nuclear magnetic resonance; Vero cell line

Citation Information

Scopus Citations: 2

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