In vivo evaluation of anticryptosporidial effects of wheat germ extracts in immunocompromised mice

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdelmaksoud H.F.; Osman E.E.A.; Abdel-Hameed S.S.; Aboushousha T.; Naggar H.M.E.

Journal: Journal of Parasitic Diseases

Publisher: Springer

Publication Date: 18 June 2022

Volume / Issue: Volume 46 / Issue 3

Pages: 833–844

ISSN: 9717196

DOI: 10.1007/s12639-022-01502-5

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Green Open Access

Authors and Affiliations

Abdelmaksoud H.F., Department of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt; Osman E.E.A., Department of Medicinal Chemistry, Theodor Bilharz Institute, Giza, Egypt; Abdel-Hameed S.S., Department of Medicinal Chemistry, Theodor Bilharz Institute, Giza, Egypt; Aboushousha T., Department of Pathology, Theodor Bilharz Research Institute, Giza, Egypt; Naggar H.M.E., Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Abstract

Cryptosporidium species is a prime cause of diarrheal disease in individuals with competent immunity. In patients with compromised immunity, infections are more severe particularly in developing countries. Wheat germ oil was described to have antiparasitic effect. This study was done to evaluate the possible role of wheat germ extracts in Cryptosporidium parvum (C. parvum) infected immunocompromised mice. Thirty white albino mice were classified into six groups as follow: four study groups, all immunosuppressed and infected with C. parvum oocysts. These four groups received treatments as follow: Group (I): treated with nitazoxanide. Group (II): treated with wheat germ oil. Group (III): treated with wheat germ extracted by hexane. Group (IV): treated with wheat germ extracted by ethanol. The remaining two groups were immunosuppressed control groups as follow: Group (V): only infected with C. parvum oocysts (Positive control). Group (VI): non-infected (Negative control). Stool samples were collected and examined to detect oocyst and the ileocecal region was subjected to histopathological and immunohistochemical examination. Wheat germ extracts showed a statistically significant effect against C. parvum specially wheat germ oil with P value: < 0.001, this effect was also confirmed by pathological and immunohistochemical examinations. C. parvum has an influence on human health by its effect in diarrheal disease. Wheat germ oil and its extracts has proved to be a reliable herb for C. parvum. treatment confirmed by different methodologies. © 2022, Indian Society for Parasitology.

Keywords

Cryptosporidium; Immunocompromised; Nitazoxanide; Wheat germ; alcohol; anticryptosporidial; antiparasitic agent; bovine serum albumin; caspase 3; citrate sodium; dexamethasone; diaminobenzidine; essential oil; hexane; hydrogen peroxide; transcription factor Cdx2; unclassified drug; animal experiment; animal model; antigen retrieval; Article; cell proliferation; controlled study; cryptosporidiosis; Cryptosporidium parvum; developing country; diarrhea; feces analysis; herb; histopathology; human; immunity; immunohistochemistry; immunosuppressive treatment; in vivo study; intestine crypt; mouse; nonhuman; oocyst; parasitology; phylogeny; pilot study; protein expression; quantitative analysis; sanitation; thickness

Citation Information

Scopus Citations: 0

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