Antioxidant and anti-aging effects of Warburgia salutaris bark aqueous extract: Evidences from in silico, in vitro and in vivo studies

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdelfattah M.A.O.; Dmirieh M.; Ben Bakrim W.; Mouhtady O.; Ghareeb M.A.; Wink M.; Sobeh M.

Journal: Journal of Ethnopharmacology

Publisher: Elsevier Ireland Ltd

Publication Date: June 2022

Volume / Issue: Volume 292

Article No.: 115187

ISSN: 3788741

DOI: 10.1016/j.jep.2022.115187

Scopus: View on Scopus

PubMed: 35288287

Document Type: Article

Access: All Open Access; Hybrid Gold Open Access

Authors and Affiliations

Abdelfattah M.A.O., College of Engineering and Technology, American University of the Middle East, Kuwait; Dmirieh M., Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg, 69120, Germany; Ben Bakrim W., AgroBioSciences, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, Ben-Guerir, 43150, Morocco, African Sustainable Agriculture Research Institute (ASARI), Mohammed VI Polytechnic University (UM6P), Laâyoune, Morocco; Mouhtady O., College of Engineering and Technology, American University of the Middle East, Kuwait; Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornish El-Nile, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Wink M., Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg, 69120, Germany; Sobeh M., AgroBioSciences, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, Ben-Guerir, 43150, Morocco

Abstract

Ethnopharmacological relevance: The genus Warburgia (family Canellaceae) is widely distributed over Afrotropical and Neotropical realms. Traditionally, W. salutaris (G. Bertol.) Chiov., and other Warburgia species are used as anti-inflammatory, antimalarial, antimicrobial, and for wound healing, and treating several skin complaints as well. Specifically, different extracts from W. salutaris were reported to possess diuretic, anti-inflammatory, and cytotoxic effects. Aim of the study: This work aimed to investigate the phytochemical composition of an aqueous extract from W. salutaris bark, and evaluate its antioxidant and anti-aging activities in silico, in vitro, and in vivo. Materials and methods: HPLC-PDA-MS/MS was used to investigate the phytochemical components of the extract. The antioxidant potential of the extract was evaluated in vitro using DPPH and FRAP assays. The Caenorhabditis elegans nematodes model was adopted to investigate the antioxidant and the anti-aging effects in vivo by determining the worms' survival rate, level of ROS, HSP16 expression, and nuclear translocation of the transcription factor DAF16. Molecular operating environment (MOE) software was utilized for in silico molecular docking of the extract's components into different enzymes involved in the aging process. Anti-collagenase, anti-elastase, anti-tyrosinase, and anti-hyaluronidase assays were used to evaluate the anti-aging effects in vitro. Results: HPLC-MS analysis furnished 30 compounds, among them catechin, 11α-hydroxy muzigadiolide, mukaadial, pereniporin B, and 11α-hydroxycinnamosmolide. The major components of the extract showed appropriate fitting in the binding site of the target enzymes adopted in the study with considerable minimum free binding energy relative to the standard inhibitors. The extract showed substantial in vitro antioxidant activity in DPPH and FRAP assays and in vitro anti-aging assays against collagenase, elastase, tyrosinase, and hyaluronidase with comparable IC50 values to the reference standards. Moreover, it attenuated oxidative stress in vivo as it significantly increased the survival rate of ROS stressed C. elegans worms, decreased intracellular ROS, decreased the juglone-induced HSP16 expression and enhanced the nuclear localization of DAF16 in a dose-dependent manner. Conclusion: Our results support the traditional use of W. salutaris to counteract inflammation and oxidative stress associated with several pathological conditions. In addition, W. salutaris bark extract can be considered as a substantial source for bioactive metabolites with strong potential as anti-aging and antioxidant agents. © 2022 The Authors

Keywords

Aging; Antioxidant; Polyphenols; Sesquiterpenoids; Warburgia salutaris; Animals; Antioxidants; Caenorhabditis elegans; Magnoliopsida; Molecular Docking Simulation; Phytochemicals; Plant Bark; Plant Extracts; Reactive Oxygen Species; Tandem Mass Spectrometry; catechin; collagenase; elastase; hyaluronidase; juglone; monophenol monooxygenase; phytochemical; plant extract; polyphenol; reactive oxygen metabolite; sesquiterpenoid; transcription factor DAF 16; animal experiment; animal model; antiaging activity; antioxidant activity; aqueous solution; Article; bark; binding site; computer model; controlled study; ferric reducing antioxidant power assay; high performance liquid chromatography; IC50; in vitro study; in vivo study; inflammation; liquid chromatography-mass spectrometry; molecular docking; nonhuman; oxidative stress; survival rate; angiosperm; animal; chemistry; metabolism

Citation Information

Scopus Citations: 42

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