Effects of Desflurane exposure and Laparotomy on genomic biomarkers and hepatic histopathology in an experimentally induced liver injury model: A pilot study

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Khafagy H.F.; AbuSeada A.N.; Shash A.M.; Elayashy M.; El-Araby R.E.; Sabry O.M.; Montasser A.Y.; Mohamed M.S.; Ebied R.S.; Samhan Y.M.

Journal: Egyptian Journal of Anaesthesia

Publisher: Taylor and Francis Ltd.

Publication Date: 25 April 2022

Volume / Issue: Volume 38 / Issue 1

Pages: 242–248

ISSN: 11101849

DOI: 10.1080/11101849.2022.2069219

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Hybrid Gold Open Access

Authors and Affiliations

Khafagy H.F., Department of Anesthesia and Surgical Intensive Care, Theodor Bilharz Research Institute, Ministry of Higher Education and Scientific Research, Giza, Egypt; AbuSeada A.N., Department of Anesthesia and Surgical Intensive Care, Theodor Bilharz Research Institute, Ministry of Higher Education and Scientific Research, Giza, Egypt; Shash A.M., Department of Anesthesia, Faculty of Medicine, Cairo University, Ministry of Higher Education and Scientific Research, Cairo, Egypt; Elayashy M., Department of Anesthesia, Faculty of Medicine, Cairo University, Ministry of Higher Education and Scientific Research, Cairo, Egypt; El-Araby R.E., Molecular Biology, Central Lab, Theodor Bilharz Research Institute, Ministry of Higher Education and Scientific Research, Giza, Egypt; Sabry O.M., Department of Hematology, Theodor Bilharz Research Institute, Ministry of Higher Education and Scientific Research, Giza, Egypt; Montasser A.Y., Department of Pathology, Theodor Bilharz Research Institute, Ministry of Higher Education and Scientific Research, Giza, Egypt; Mohamed M.S., Experimental Laboratory Unit Theodor Bilharz Research Institute, Ministry of Higher Education and Scientific Research, Giza, Egypt; Ebied R.S., Department of Anesthesia and Surgical Intensive Care, Theodor Bilharz Research Institute, Ministry of Higher Education and Scientific Research, Giza, Egypt; Samhan Y.M., Department of Anesthesia and Surgical Intensive Care, Theodor Bilharz Research Institute, Ministry of Higher Education and Scientific Research, Giza, Egypt

Abstract

Background: Halogenated anesthetics are known to cause hepatotoxicity that can be evaluated by the emerging genomic biomarkers, that is, miRNA-122 and miRNA-192. Methods: Thirty Wister rats were distributed randomly in five equal groups; Control Group (group C); Desflurane Group (group D); Hypoxia Group (group H); Hypoxia + Desflurane Group (group HD); and Hypoxia + Desflurane + Laparotomy Group (group HDL). Animals in the Groups D, HD, HDL were exposed to 6% desflurane. Rats were exposed to hypoxic mixture in Groups H, HD, and HDL. Animals in Group HDL were additionally subjected to laparotomy with liver palpation. After 24 hours, plasma ALT, AST, GGT, blood and tissue miR-122, and miR-192 gene expression and histopathological examination of liver biopsies were performed. Results: Plasma and tissue miR122 and miR192 showed significant increase ascendingly in H, D, HD, HDL groups (p value <0.05), significant elevation of ALT, AST, GGT in HD and HDL groups (p value <0.05). Hepatic histopathological findings revealed 33.3% inflammatory infiltration in group D, 50% in group H, 66.6% in HD and HDL groups. 16.7% apoptosis was detected in D & H groups and 33.3% in HD and HDL groups, while none showed necrosis. Conclusion: This experimental study concluded that each desflurane and hypoxia has deleterious effect on hepatic integrity, which increased by their combination and aggravated by surgical influence as verified by miR-122 and miR-192, as well as histopathological examination. Assessment of hepatotoxic effects of other various anesthetics and different surgical approaches in experimental study using hepatic genomic biomarkers is recommended. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

Desflurane; histopathology; laparotomy; liver injury model; MiRNA; alanine aminotransferase; aspartate aminotransferase; gamma glutamyltransferase; high density lipoprotein; microRNA 122; microRNA 192; phenobarbital; alanine aminotransferase blood level; animal experiment; animal model; animal tissue; Article; aspartate aminotransferase blood level; controlled study; experimental liver injury; gamma glutamyl transferase blood level; gene expression; genetic marker; liver function test; male; nonhuman; outcome assessment; rat; real time polymerase chain reaction; RNA extraction

Citation Information

Scopus Citations: 0

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