Vancomycin-Loaded Furriness Amino Magnetic Nanospheres for Rapid Detection of Gram-Positive Water Bacterial Contamination

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Azzam A.M.; Shenashen M.A.; Selim M.S.; Mostafa B.; Tawfik A.; El-Safty S.A.

Journal: Nanomaterials

Publisher: MDPI

Publication Date: 1 February 2022

Volume / Issue: Volume 12 / Issue 3

Article No.: 510

ISSN: 20794991

DOI: 10.3390/nano12030510

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Azzam A.M., National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba-shi, Ibaraki-ken, 305-0047, Japan, Environmental Research Department, Theodor Bilharz Research Institute (TBRI), Imbaba, Giza, 12411, Egypt; Shenashen M.A., National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba-shi, Ibaraki-ken, 305-0047, Japan, Egyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, 11727, Egypt; Selim M.S., National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba-shi, Ibaraki-ken, 305-0047, Japan, Egyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, 11727, Egypt; Mostafa B., Environmental Research Department, Theodor Bilharz Research Institute (TBRI), Imbaba, Giza, 12411, Egypt; Tawfik A., Water Pollution Research Department, National Research Centre (NRC), Dokki, Giza, 12622, Egypt; El-Safty S.A., National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba-shi, Ibaraki-ken, 305-0047, Japan

Abstract

Bacterial pathogens pose high threat to public health worldwide. Different types of nano-materials have been synthesized for the rapid detection and elimination of pathogens from environmental samples. However, the selectivity of these materials remains challenging, because target bacterial pathogens commonly exist in complex samples at ultralow concentrations. In this study, we fabricated novel furry amino magnetic poly-L-ornithine (PLO)/amine-poly(ethylene glycol) (PEG)-COOH/vancomycin (VCM) (AM-PPV) nanospheres with high-loading VCM for vehicle tracking and the highly efficient capture of pathogens. The magnetic core was coated with organosilica and functionalized with cilia. The core consisted of PEG/PLO loaded with VCM conjugated to Gram-positive bacterial cell membranes, forming hydrogen bonds with terminal peptides. The characterization of AM-PPV nanospheres revealed an average particle size of 56 nm. The field-emission scan-ning electron microscopy (FE-SEM) micrographs showed well-controlled spherical AM-PPV nano-spheres with an average size of 56 nm. The nanospheres were relatively rough and contained an additional 12.4 nm hydrodynamic layer of PLO/PEG/VCM, which provided additional stability in the suspension. The furry AM-PPV nanospheres exhibited a significant capture efficiency (>90%) and a high selectivity for detecting Bacillus cereus (employed as a model for Gram-positive bacteria) within 15 min, even in the presence of other biocompatible pathogens. Moreover, AM-PPV nano-spheres rapidly and accurately detected B. cereus at levels less than 10 CFU/mL. The furry nano-design can potentially satisfy the increasing demand for the rapid and sensitive detection of pathogens in clinical and environmental samples. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Bacterial pollution; Detection; Magnetic nanospheres; Vancomycin

Citation Information

Scopus Citations: 13

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