Conjugated silver nanoparticles as a diagnostic tool for circulating hydatid antigens

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Shakra M.Y.; Abou-Sheishaa G.A.; Hafez A.O.; Shalash I.R.

Journal: The Egyptian journal of immunology

Publisher: NLM (Medline)

Publication Date: 2022

Volume / Issue: Volume 29 / Issue 4

Pages: 84–93

ISSN: 11104902

Scopus: View on Scopus

PubMed: 36198106

Document Type: Article

Authors and Affiliations

Shakra M.Y., Department of Medical Parasitology, Damietta Faculty of Medicine, Al-Azhar University, Egypt; Abou-Sheishaa G.A., Department of Medical Parasitology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt; Hafez A.O., Department of Medical Parasitology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt; Shalash I.R., Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Cystic hydatid disease is one of the most significant worldwide zoonotic diseases. The causative agent is the larval stage of Echinococcus granulosus. The diagnosis of cystic echinococcosis by clinical symptoms and scanning alone is often difficult and confusing. The definite diagnosis needs sensitive and reliable serological tests. This study aimed to evaluate a nano silver-based enzyme linked immunosorbent assay (ELISA) in the detection of circulating hydatid antigen in human serum samples. The study included 66 human serum samples (36 hydatidosis confirmed cases, 15 cases infected with other parasites, and 15 normal subjects as negative control). The circulating protoscolices antigen was assayed by the nano-silver dot ELISA, nano-silver sandwich ELISA and the traditional methods (dot ELISA and sandwich ELISA). Our study revealed that the sensitivity and specificity of the nano-silver dot ELISA were 97.2% and 93.3%, respectively. The sensitivity and specificity of nano-silver sandwich ELISA were 94.4% and 96.7% respectively. The nano-silver-based ELISA showed higher sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy than the traditional ELISA. In conclusion, the nano-silver-based ELISA can be proposed as a confirmatory test in the diagnosis of cystic echinococcosis. Copyright© by the Egyptian Association of Immunologists.

Keywords

Antibodies, Helminth; Antigens, Helminth; Echinococcosis; Enzyme-Linked Immunosorbent Assay; Humans; Metal Nanoparticles; Sensitivity and Specificity; Silver; helminth antibody; metal nanoparticle; parasite antigen; enzyme linked immunosorbent assay; human; procedures

Citation Information

Scopus Citations: 4

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