NAFLD mark: an accurate model based on microRNA-34 for diagnosis of non-alcoholic fatty liver disease patients

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Mohamed A.A.; El-Demery A.; Al-Hussain E.; Mousa S.; Halim A.A.; Mostafa S.M.; Abdelghany R.S.; Mahmoud S.M.; Elkady M.A.; Raafat K.; Hassnine A.A.; Omran M.M.

Journal: Journal of Genetic Engineering and Biotechnology

Publisher: Springer Science and Business Media Deutschland GmbH

Publication Date: December 2021

Volume / Issue: Volume 19 / Issue 1

Article No.: 157

ISSN: 1687157X

DOI: 10.1186/s43141-021-00257-5

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Mohamed A.A., Biochemistry Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt; El-Demery A., Biochemistry Department, Faculty of Medicine, October 6 University, 6th of October City, Egypt; Al-Hussain E., Clinical and Chemical Pathology, Faculty of Medicine, Cairo university, Giza, Egypt; Mousa S., Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Halim A.A., Tropical Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt; Mostafa S.M., Tropical Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt; Abdelghany R.S., Tropical Medicine Department, Ahmed Maher Teaching Hospital, Cairo, Egypt; Mahmoud S.M., Tropical Department, El-Sahel Teaching Hospital, Cairo, Egypt; Elkady M.A., Theodor Bilharz Research Institute Gastroenterology and Hepatology department, Cairo, Egypt; Raafat K., Gastroenterology and Hepatology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Hassnine A.A., Department of Gastroenterology and Tropical Medicine, Faculty of Medicine, Minia University, Minia, Egypt; Omran M.M., Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, 11795, Egypt

Abstract

Background: It remains essential for non-alcoholic fatty liver (NAFLD) patients, to develop a sensitive and specific diagnostic model. Data regarding the use of micro (mi)RNA-34 for NAFLD diagnosis are few. Routine clinical assessment, laboratory tests were done for Egyptian individuals (n = 314) were included (100 healthy individuals and 214 NAFLD patients). Quantification of miRNA-34 was done using real-time PCR. Extremely significant variables were entered into stepwise logistic regression. The diagnostic power of variables was estimated by the area under the ROC (AUC). Results: MiRNA-34 levels were higher in NAFLD patients than healthy individuals with a significant difference (P< 0.0001). The multivariate analysis was used to evaluate the NAFLD-associated variables (CRP, cholesterol, body mass index (BMI), ALT had p< 0.0001 while mRNA-34 had (p=0.0004). The AUCs (CI) of candidate NAFLD markers were in the order of miRNA-34 0.72 (0.66–0.77) < ALT 0.73 (0.67–0.79) < BMI 0.81 (0.76–0.86) < cholesterol < 0.85 (0.79–0.90) < CRP 0.88 (0.84–0.92). We developed a novel index for discriminating patients with NAFLD named NAFLD Mark. AUC was jumped to 0.98 (0.93–0.99) when five markers were combined. The AUC of NAFLD mark for NAFLD detection was higher than the AUCs of seven common NAFLD indexes (0.44–0.86). Conclusions: The NAFLD mark is a non-invasive and highly sensitive and specific model for NAFLD diagnosis. © 2021, The Author(s).

Keywords

Alanine aminotransferase; Body mass index; C-reactive protein; Cholesterol; MicroRNA-34; Non-alcoholic fatty liver disease

Citation Information

Scopus Citations: 8

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