Evaluation of Annona muricata (Graviola) leaves activity against experimental trichinellosis: In vitro and in vivo studies

Theodor Bilharz Research Institute

Bibliographic Information

Authors: El-Wakil E.S.; Abdelmaksoud H.F.; Aboushousha T.S.; Ghallab M.M.I.

Journal: Journal of Helminthology

Publisher: Cambridge University Press

Publication Date: 13 September 2021

Volume / Issue: Volume 95

Article No.: e53

ISSN: 0022149X

DOI: 10.1017/S0022149X21000481

Scopus: View on Scopus

PubMed: 34515021

Document Type: Article

Authors and Affiliations

El-Wakil E.S., Department of Parasitology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt; Abdelmaksoud H.F., Department of Parasitology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt; Aboushousha T.S., Department of Pathology, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza, 12411, Egypt; Ghallab M.M.I., Department of Medical Parasitology, Kafrelsheikh Faculty of Medicine, Kafrelsheikh University, ElGiesh street, Kafrelsheikh Governorate, 33516, Egypt

Abstract

Our work aimed to evaluate the possible effect of Annona muricata (Graviola) leaf extract on Trichinella spiralis in in vitro and in vivo studies. Trichinella spiralis worms were isolated from infected mice and transferred to three culture media - group I (with no drugs), group II (contained Graviola) and group III (contained albendazole) - then they were examined using the electron microscope. In the in vivo study, mice were divided into five groups: GI (infected untreated), GII (prophylactically treated with Graviola for seven days before infection), GIII (infected and treated with Graviola), GIV (infected and treated with albendazole) and GV (infected and treated with a combination of Graviola plus albendazole in half doses). Drug effects were assessed by adults and larvae load beside the histopathological small intestinal and muscular changes. A significant reduction of adult and larval counts occurred in treated groups in comparison to the control group. Histopathologically, marked improvement in the small intestinal and muscular changes was observed in treated groups. Also, massive destruction of the cultured adults' cuticle was detected in both drugs. This study revealed that Graviola leaves have potential activity against trichinellosis, especially in combination with albendazole, and could serve as an adjuvant to anti-trichinellosis drug therapy. Copyright © The Author(s), 2021. Published by Cambridge University Press.

Keywords

albendazole; Annona muricata (Graviola); electron microscopy; mice; prophylactic; Trichinella spiralis; Animals; Annona; Disease Models, Animal; Trichinellosis; Annona muricata extract; animal cell; animal experiment; animal model; Annona muricata; antifungal activity; antifungal therapy; Article; controlled study; drug efficacy; drug screening; histopathology; in vitro study; in vivo study; male; mouse; nonhuman; plant leaf; scanning electron microscopy; skeletal muscle; trichinosis; worm burden; animal; disease model

Citation Information

Scopus Citations: 18

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