The protective role of diosmin, hesperidine combination against heavy metals toxicity in wistar albino rats: Biochemical, immunohistochemical and molecular studies

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Sarhan H.K.A.; Saleh A.M.A.; Hammam O.A.E.; Atta A.H.; El-Nehrery I.M.

Journal: Egyptian Journal of Chemistry

Publisher: NIDOC (Nat.Inform.Document.Centre)

Publication Date: 1 August 2021

Volume / Issue: Volume 64 / Issue 8

Pages: 4531–4543

ISSN: 4492285

DOI: 10.21608/ejchem.2021.83002.4078

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Bronze Open Access

Authors and Affiliations

Sarhan H.K.A., Medical and Radiation Research Department, Research Sector, Nuclear Material Authority, Cairo, Egypt; Saleh A.M.A., Head of Giza scientific Office, Egyphar for Pharmaceutical Idustry, El-Obbore, Egypt; Hammam O.A.E., Pathology Department, Thiodor Bellharz Inistitute for Research, Ministry of Scientific Research, Cairo, Egypt; Atta A.H., Chemistry Department, Faculty of Science, Suiez University, Suiez, Egypt; El-Nehrery I.M., Chemistry Department, Faculty of Science, Suiez University, Suiez, Egypt

Abstract

The benchmark of this study is to evaluate the antioxidant protective efficiency of Diosmin-Hesperidin combination, a natural citrus flavone of hesperidin derivative on heavy metals intoxication and Oxidative stress-induced damage in Wistar albino rats. Oral doses of diosmin-hesperidin in rats (200 and 100 mg/kg body weight, respectively) for a month (every other day) prior to heavy metals intoxication. Evaluation of the protective and antioxidant effects of the combination of diosmin and hesperidin, various Rt-PCR estimations, biochemical estimations, histopathological alterations as well as comet assay and caspase-3 activity for assessment of apoptosis were performed. Results indicated that heavy metals intoxication-induced decline in the levels of liver tissue P53 gene expression and increase of liver tissues of the apoptotic caspase-3 gene, also induced decline in the levels of liver tissue antioxidant parameters (SOD, GPx, and GSH), increased lipid peroxidation (MDA), DNA damage and apoptosis, these parameters were improved by pre-administration of diosmin+hesperidine. Diosmin+hesperidine dose (200 and100 mg/kg body wt. respectively) restored the p53 and caspase-3 genes near-normal values, antioxidant status to near normal and reduced lipid peroxidation, DNA, and tissue damage. These results were confirmed by histopathological examinations, which showed that pre-administration of diosmin+hesperidine protected the liver of albino rats against heavy metals intoxication-induced damage. Hence, it has been illustrated that diosmin+hesperidine might be an effective antioxidant and protector against heavy metals intoxication-induced damage in rats. Moreover, the diosmin+hesperidine alone pretreated group did not show any biochemical alterations, fold change of P53 or caspase-3 genes or DNA damage indicating the protective nature of the drug. © 2021 National Information and Documentation Center (NIDOC)

Keywords

Antioxidants; Apoptosis; Diosmin+Hesperidine combination; DNA damage; Heavy metals intoxication; Histopathology; Reactive oxygen species; Rt-PCR

Citation Information

Scopus Citations: 7

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