Comparative Study between Wild and Commercial Egyptian Lactobacillus reuteri-Antagonizing Effects in Cryptosporidiosis Models

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Fahmy A.; AbuelenAin G.; Abdou A.; Elhakeem M.

Journal: Advances in Animal and Veterinary Sciences

Publisher: Nexus Academic Publishers

Publication Date: April 2021

Volume / Issue: Volume 9 / Issue 6

Pages: 802–810

ISSN: 23093331

DOI: 10.17582/journal.aavs/2021/9.6.802.810

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Fahmy A., Department of Immunology and Drug Evaluation, TBRI, Giza, Egypt, FCHS, IAT, P.O. Box 111534, United Arab Emirates; AbuelenAin G., Department of Immunology and Drug Evaluation, TBRI, Giza, Egypt, FCHS, IAT, P.O. Box 111534, United Arab Emirates; Abdou A., Department of Microbiology and Immunology, NRC, Giza, Egypt; Elhakeem M., Water Resources and Environmental Engineering, ADU, Abu Dhabi, United Arab Emirates, University of Tennessee, Knoxville, TN, United States

Abstract

To investigate the prophylactic and therapeutic potentiality of Egyptian probiotic Lactobacillus reuteri in the cryptosporidiosis model (hereafter home-isolated probiotic). As per the health conditions of the experimental mice, they were deployed into two main batches: immunocompetent and immunocompromised. Each batch comprised three protocols as per the types of the treatment (home-isolated-, commercial probiotics, and Nitazoxanide). A dose of 108 colony-forming unit (cfu) Lactobacillus reuteri isolated from the domestic camels and the commercial strain were administered orally on daily basis for 10 days. Then, mice were infected with approximately 1 x 104 inoculums of Cryptosporidium cysts with continuous administration of probiotics till the end of the experiment (day 20). The regression decay models were utilized to predict the recovery rate of the experimental mice at days different from the tested ones using the data collected from the Nitazoxanide-different time-scheduled protocol. The response to the treatment by probiotics in the immunocompromised mice is less than that of the immunocompetent mice. Positive outcome results are in favor of the home-isolated probiotic treatment. On the contrary, Nitazoxanide is the treatment with the highest number of cysts recovered from the experimental mice. In a nutshell, the results demonstrate that the home-isolated probiotic is efficient in decolonizing the Cryptosporidium spp. cysts and ameliorating the pathogenicity of the disease. Besides, this research study is shedding the light on the importance of isolating and utilizing a country/region-based Lactobacillus reuteri probiotic for high-efficiency treatment of cryptosporidiosis in immunodeficient cases. © 2021 Abuelenain et al. All Rights Reserved.

Keywords

Cryptosporidiosis; Drug discovery; Nitazoxanide; Probiotic

Citation Information

Scopus Citations: 5

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