Serum low replacement medium versus serum rich replacement medium for production of Anti-Schistosoma Monoclonal antibody: A comparative study

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Mahmoud F.S.; Demerdash Z.A.; Elmotawakel S.M.; Hassan S.; Hendawy M.; Atta S.A.; Shawky S.M.; Alkhateeb E.M.; Hassanin H.I.

Journal: Clinical Epidemiology and Global Health

Publisher: Elsevier B.V.

Publication Date: June 2020

Volume / Issue: Volume 8 / Issue 2

Pages: 423–427

ISSN: 22133984

DOI: 10.1016/j.cegh.2019.09.014

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Hybrid Gold Open Access

Authors and Affiliations

Mahmoud F.S., Immunology Department, Theodor Bilharz Research Institiute, Egypt; Demerdash Z.A., Immunology Department, Theodor Bilharz Research Institiute, Egypt; Elmotawakel S.M., Immunology Department, Theodor Bilharz Research Institiute, Egypt; Hassan S., Immunology Department, Theodor Bilharz Research Institiute, Egypt; Hendawy M., Parasitology Department, Theodor Bilharz Research Institute, Egypt; Atta S.A., Immunology Department, Theodor Bilharz Research Institiute, Egypt; Shawky S.M., Clinical and Chimical Pathology Department, Faculty of Medcine, CairoUniversity, Egypt; Alkhateeb E.M., Clinical and Chimical Pathology Department, Faculty of Medcine, CairoUniversity, Egypt; Hassanin H.I., Immunology Department, Theodor Bilharz Research Institiute, Egypt

Abstract

Objective: to assess the production of Anti-Schistosoma Monoclonal Antibodies by growing IgM producing hybridoma cell line in serum low medium and to evaluate their diagnostic potential to replace the old conventional method in animal ascetic fluid. Methods: Highly reactive and specific IgM MAb secreting hybridoma cell line to S. mansoni SEA was cultured in three different media; serum rich replacement medium (conventional), serum low replacement medium (SLRM) and serum free replacement medium (SFRM). Results: Cell count and viability were better in conventional medium and SLRM than SFRM. The lower detection limit of the assay Mab of SLRM culture was 1.1 ng/ml of S. mansoni SEA. SFRM was excluded because of the low concentration and reactivity. MAb was tested against 34 human samples. Sensitivity and specificity of the assay for each were 92% and specificity 90% for SLRM respectively. The diagnostic efficacy of the assay was 94%. Conclusions: the production and diagnostic efficacy of antischistosomal MAbs was comparable on usage of either in vitro cell culture supernate in SLRM or conventional media. © 2019

Keywords

Hybridoma; Monoclonal antibody; Replacement media; Schistosomiasis; Soluble egg antigen; antigen; antischistosomal agent; immunoglobulin M; antibody production; Article; cell count; cell viability; comparative study; controlled study; enzyme linked immunosorbent assay; false negative result; false positive result; human; hybridoma cell culture; hybridoma cell line; limit of detection; nonhuman; priority journal; Schistosoma mansoni; sensitivity and specificity

Citation Information

Scopus Citations: 2

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