Chemical profiles and bio-activities of different extracts of terfezia species and their other associated fungi

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Ghareeb M.A.; Khalaf O.M.; Abdel-Aziz M.S.; Saad A.M.; Madkour H.M.F.; El-Ziaty A.K.; Refahy L.A.

Journal: Current Bioactive Compounds

Publisher: Bentham Science Publishers

Publication Date: 10 June 2020

Volume / Issue: Volume 16 / Issue 3

Pages: 308–319

ISSN: 15734072

DOI: 10.2174/1573407214666181009110805

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Ghareeb M.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, 12411 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt; Khalaf O.M., Chemistry Department, Faculty of Science, Ain Shams University, El-Khalifa El-Mamoun, Abbassia, Cairo, 11566, Egypt; Abdel-Aziz M.S., Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, El Behoos Street 33, Dokki, Giza, 12622, Egypt; Saad A.M., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, 12411 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt; Madkour H.M.F., Chemistry Department, Faculty of Science, Ain Shams University, El-Khalifa El-Mamoun, Abbassia, Cairo, 11566, Egypt; El-Ziaty A.K., Chemistry Department, Faculty of Science, Ain Shams University, El-Khalifa El-Mamoun, Abbassia, Cairo, 11566, Egypt; Refahy L.A., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, 12411 Warrak El-Hadar, Imbaba (P.O. 30), Giza, Egypt

Abstract

Background: Desert truffles (Terfezia species) are known by their vital nutritional benefits as they are considered as rich sources of vitamins, fatty acid, minerals and proteins. Methods: The chemical constituents of the different solvent extracts of Terfezia species were isolated and identified by column chromatography, spectroscopic and GC-MS analyses. Also, the ethyl acetate and acetone extracts of different fungal isolates, associated Terfezia, after grown on rice medium were screened for their antimicrobial, anticancer and antioxidant activities via disc agar plate, micro culture tetrazolium (MTT) and 2,2-azino-di-[3-ethylbenzo-thiazolin-sulphonate] (ABTS) assays, respectively. The promising fugal strains were molecularly identified by 18SrRNA tool. Results: Bio-guided separation of methylene chloride ethyl acetate and n-butanol fractions of Terfezia species led to identification of nine compounds namely; (R)-4,8-dihydroxy-7-hydroxymethyl-6-methoxy isochroman-1-one (1), 4-deoxy-4α-phorbal-12-(2,3-dimethyl)butyrate-13-isobutyrate (2), oxy-phylline B (3), terfezien A (4), latilagascene D (5), amaiouine (6), senbusine acetate (7), terfezien B (8) and marinoquinoline D (9). Moreover, sixteen compounds were identified in the n-hexane extract via GC-MS analysis, accounting for 93.69% of the total detected components in the extract. While, twenty five components were detected in the methylene chloride extract, representing 43.86% from total detected components in the extract. Eight fungal strains were isolated from Terfezia sp., powder by serial dilution methods and these fungi were cultivated on solid rice medium. Also, their ethyl acetate and acetone extracts were subjected to biological studies including antimicrobial, antioxidant and anticancer activities. The three potent fungal strains (1M, 4M and 8M) were identified by the molecular technique 18SrRNA as Aspergillus niger 1M-EGY-IQ, Penicillium crustosum 4M-EGY-IQ, and Fusarium prolif-eratum 8M-EGY-IQ for 1M, 4M and 8M, respectively. Conclusion: Terfezia sp., comprise a rich source of bioactive compounds and could be considered as an interesting candidate for the treatment of infectious diseases. © 2020 Bentham Science Publishers.

Keywords

Bio-activities; Fungal isolation; GC-MS; Molecular identification; Secondary metabolites; Terfezia species; 2,4 di tert butylphenol; 4 deoxy 4alpha phorbal 12 (2,3 dimethyl)butyrate 13 isobutyrate; 4,8 dihydroxy 7 hydroxymethyl 6 methoxy isochroman 1 one; acetic acid ethyl ester; acetone; antiinfective agent; antineoplastic agent; antioxidant; ascorbic acid; benzaldehyde,4 hydroxy; butanol; cycloheximide; dichloromethane; doxorubicin; fungal extract; hexacosa 3,7 diene; hexane; latilagascene d; linoleic acid; marinoquinoline d; neomycin; nonanal; oxyphylline b; palmitic acid methyl ester; pentadecanoic acid,14 methyl methyl ester; senbusine acetate; Terfezia extract; terfezien a; terfezien b; unclassified drug; unindexed drug; antimicrobial activity; antineoplastic activity; antioxidant activity; Article; Aspergillus niger; chemical composition; column chromatography; controlled study; DNA sequencing; edible alga; fungal strain; fungus spore; Fusarium proliferatum; HCT 116 cell line; Hep-G2 cell line; human; human cell; mass fragmentography; MCF-7 cell line; nonhuman; nuclear magnetic resonance spectroscopy; Penicillium crustosum; proton nuclear magnetic resonance; Terfezia

Citation Information

Scopus Citations: 7

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