Detection of high level aminoglycoside resistance genes among clinical isolates of Enterococcus species

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Diab M.; Salem D.; El-Shenawy A.; El-Far A.; Abdelghany A.; Awad A.R.; El Defrawy I.; Shemis M.

Journal: Egyptian Journal of Medical Human Genetics

Publisher: Springer

Publication Date: 27 November 2019

Volume / Issue: Volume 20 / Issue 1

Article No.: 28

ISSN: 11108630

DOI: 10.1186/s43042-019-0032-3

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Diab M., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, 12511, Egypt; Salem D., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, 12511, Egypt; El-Shenawy A., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, 12511, Egypt; El-Far A., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, 12511, Egypt; Abdelghany A., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Awad A.R., Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; El Defrawy I., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, 12511, Egypt; Shemis M., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt

Abstract

Background: Enterococci are intrinsically resistant to clinically achievable concentrations of aminoglycosides. However, high-level resistance to aminoglycosides (HLAR) is primarily due to the acquisition of genes encoding aminoglycoside-modifying enzymes (AMEs). Aminoglycosides along with cell wall inhibitors are given clinically for treating enterococcal infections. The current study was conducted to investigate the rate of HLAR and to determine aminoglycoside resistance encoding genes profile in enterococcal isolates from different clinical specimens. Results: From 120 Enterococcus species, 50 (41.7%) enterococcal isolates were proven to have HLAR, 78% (39/50) have high-level gentamicin resistance (HLGR), and 74% (37/50) were high-level streptomycin-resistant (HLSR). HLGR isolates carried aminoglycoside modifying gene aac (6′)-Ie-aph (2′)-Ia in 26/39 (66.7%) of isolates, whereas 32/37 (86.5%) of HLSR carried aph (3′)-IIIa gene and were observed in E. faecalis, E. faecium, E. gallinarum, and E. casseliflavus. The aph (2′)-Ib, aph (2′)-Ic, and aph (2′)-Id that encode HLGR could not be detected. Conclusions: The high detection rate of HLAR among the studied Enterococcus species and the coexistence of HLGR and HLSR strains provide crucial insights to the necessity of routine testing for HLAR in the microbiology lab. The main AME genes among HLGR and HLSR enterococci were aac (6′)-Ie-aph (2″)-Ia and aph (3′)-IIIa, respectively. © 2019, The Author(s).

Keywords

Aminoglycoside-modifying enzyme gene; Enterococcus species; High-level aminoglycoside resistance; aminoglycoside resistance; Article; bacterial gene; bacterium detection; bacterium isolation; controlled study; Enterococcus; Enterococcus casseliflavus; Enterococcus faecalis; Enterococcus faecium; Enterococcus gallinarum; gentamicin resistance; human; modifier gene; nonhuman; prospective study; streptomycin resistance

Citation Information

Scopus Citations: 19

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