Association of MicroRNA 196a and 499 polymorphisms with development of cirrhosis and hepatocellular carcinoma Post-HCV infection in Egyptian patients

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Fteah A.M.; Ahmed A.I.; Mosaad N.A.; Hassan M.M.; Mahmoud S.H.

Journal: Asian Pacific Journal of Cancer Prevention

Publisher: Asian Pacific Organization for Cancer Prevention

Publication Date: 1 November 2019

Volume / Issue: Volume 20 / Issue 11

Pages: 3479–3485

ISSN: 15137368

DOI: 10.31557/APJCP.2019.20.11.3479

Scopus: View on Scopus

PubMed: 31759375

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Fteah A.M., Department of Clinical and Chemical Pathology, Theodor Bilharz Research Institute, Egypt; Ahmed A.I., Department of Clinical and Chemical Pathology, Kasr Al-Ainy, Egypt; Mosaad N.A., Department of Clinical and Chemical Pathology, Kasr Al-Ainy, Egypt; Hassan M.M., Department of Clinical and Chemical Pathology, Theodor Bilharz Research Institute, Egypt; Mahmoud S.H., Department of Endemic Medicine, Liver Unit, Faculty of Medicine, Cairo University, Egypt

Abstract

Hepatocellular carcinoma (HCC) is the commonest primary tumor of the liver. Chronic HCV infection is the leading cause of end-stage liver disease, HCC and liver-related death in Egypt. Single nucleotide polymorphisms (SNPs) in microRNAs were reported to increase susceptibility to tumorigenesis; affect prognosis and as promising biomarkers in virus-host interactions. This study was conducted to investigate the role of genetic variants of miR-196a2 (rs 11614913) C>T and miR-499 (rs 3746444) A>G in the development of cirrhosis and HCC in Egyptian HCV infected patients. Genotyping of the candidate SNPs was performed by Real Time PCR in 75 HCV-related HCC patients, 75 cirrhotic patients on top of HCV and 75 healthy controls. There was significant difference in miR-499 (rs3746444) genotypes frequency between the three studied groups as the GG genotype was significantly lower in HCC cases than other groups (P = 0.009) while the combined miR-499 (AA+AG) genotypes were significantly higher in HCC cases than other groups (P = 0.005). Also a significant difference was found in miR-499 genotypes frequency when compared between HCC and cirrhosis groups as the GG genotype was significantly lower in HCC cases than cirrhosis group (P = 0.006) while the combined miR-499 (AA+AG) genotypes were significantly higher in HCC cases than in cirrhosis group (P = 0.003) [OR (95% CI) = 0.131 (0.028-0.601)]. The frequency of the G allele was significantly lower in HCC than other groups (P = 0.024) and significantly lower in HCC than normal group (P = 0.006) [OR (95%CI) = 0.501 (0.304-0.825)]. For miR-196a2 (rs11614913) C>T polymorphisms, no significant association was found with HCC risk. Our study concluded that the G allele of miR-499 is associated with lower risk of HCV related HCC development. No significant association of miR-196a2 (rs 11614913), genotypes or alleles with risk for HCC development, could be detected. © 2019, Asian Pacific Organization for Cancer Prevention.

Keywords

Hepatitis C Virus; Hepatocellular carcinoma; MiR-196a2 C>T; MiR-499 A>G; Real Time PCR; Alleles; Carcinoma, Hepatocellular; Case-Control Studies; Egypt; Female; Genetic Predisposition to Disease; Genotype; Hepacivirus; Hepatitis C; Humans; Liver Cirrhosis; Liver Neoplasms; Male; MicroRNAs; Middle Aged; Polymorphism, Single Nucleotide; microRNA; allele; case control study; genetic predisposition; genetics; human; liver cell carcinoma; liver tumor; pathogenicity; single nucleotide polymorphism; virology

Citation Information

Scopus Citations: 8

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