Effect of Interferon-Beta (IFN-β) on tumor suppressor and apoptotic markers in hepatocellular carcinoma cell line

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Okasha H.; Hassan M.; Aboushousha T.; Samir S.

Journal: International Journal of Research in Pharmaceutical Sciences

Publisher: J. K. Welfare and Pharmascope Foundation

Publication Date: 16 October 2019

Volume / Issue: Volume 10 / Issue 4

Pages: 2936–2943

ISSN: 9757538

DOI: 10.26452/ijrps.v10i4.1574

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Okasha H., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Giza, Egypt; Hassan M., Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Aboushousha T., Pathology Department, Theodor Bilharz Research Institute, Giza, Egypt; Samir S., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

IFN-β (Rebif) is a drug with valuable cancer therapeutic potential as it mediates anti-proliferation and apoptosis induction. Therefore, this study aimed to evaluate the anti-proliferation of IFN-β through assessing gene expression of p53 and caspase-3 in hepatocellular carcinoma cell (HCC) line (HepG2). The 50% inhibition viability concentration (IC50) of IFN-β was calculated by cytotoxicity assay, and it was tested on normal cell line (Vero). After treating HepG2 cells with IFN-β, p53 and caspase-3 expression was analyzed, at time intervals of 2, 4, 6, and 24 hrs, by real-time PCR, histopathology and immunohistochemistry. IC50 of IFN-β was 420 ng/ml, which wasn’t cytotoxic on normal cells. P53 expression was gradually up-regulated by time, and then, it was decreased after 24 hrs incubation. However, no expression of caspase-3 was detected compared to cell control. Histopathologically, cells degeneration was remarkable after 24 hrs while no change was noticed in control. Immunohistochemical analysis revealed a decline of p53 and caspase-3 expression in ttreated cells with IFN-β after 24 hrs, to 30% compared to cell control (75% and 60%, respectively). In conclusion, IFN-β has the potential to be utilized as a suppressor of HCC proliferation and inducer of malignant cells apoptosis. © International Journal of Research in Pharmaceutical Sciences.

Keywords

Caspase-3; Hepatocellular carcinoma; Immunohistochemistry; Interferon-beta; P53; beta interferon; caspase 3; antineoplastic activity; apoptosis; Article; cell degeneration; cell viability; controlled study; cytotoxicity; enzyme linked immunosorbent assay; gene expression; Hep-G2 cell line; hepatocellular carcinoma cell line; histopathology; human; human cell; IC50; liver cell carcinoma; protein expression; real time polymerase chain reaction; RNA extraction; selectivity index; tumor suppressor gene; upregulation; Vero cell line

Citation Information

Scopus Citations: 18

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