Characterization and evaluation of antimicrobial and cytotoxic activities of glycine max methanol extract

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdel-Hady H.; El-Wakil E.A.; Nasr S.M.

Journal: International Journal of Pharmaceutical Research

Publisher: Advanced Scientific Research

Publication Date: 1 July 2019

Volume / Issue: Volume 11 / Issue 2

Pages: 418–426

ISSN: 9752366

DOI: 10.31838/ijpr/2019.11.02.060

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

Abdel-Hady H., Medicinal Chemistry Department, Kornish El-Nile St., Warrak El-Hadder, Imbaba, Mail Box 30, P.O, Giza, 12411, Egypt; El-Wakil E.A., Medicinal Chemistry Department, Kornish El-Nile St., Warrak El-Hadder, Imbaba, Mail Box 30, P.O, Giza, 12411, Egypt; Nasr S.M., Molecular Biology Department, Theodor Bilharz Research Institute, Kornish El-Nile St., Warrak El-Hadder, Imbaba, Mail Box 30, P.O, Giza, 12411, Egypt

Abstract

Glycine max seeds use in many types of food in Egypt and all over the world. Also, it has many pharmacological properties. This study aimed to evaluate cytotoxic and antimicrobial activities of the plant extract on murine spleen cells and hepatocellular carcinoma (HepG2 cell line) as well as GC-MS analysis. The seeds were extracted with 85% methanol and tested on murine spleen cells and HepG2 cell line with different concentrations. Also, antimicrobial activity of the extract was studied by mean inhibition zone. The outcomes proved that the cytotoxic activity of the plant on murine spleen cells is very weak (380.94µg/ml) as well as on HepG2, it inhibits the cell viability% to 8.5% after 24 hrs. (IC50 = 42.5µg/ml). Also, the plant showed good antimicrobial activity in a concentration-dependent manner on fungi and bacteria (Gram positive and Gram negative). GC-MS analysis of bioactive compounds in the plant extract revealed the presence of 11 bioactive compounds. The major bioactive compounds are 9,12-octadecadienoic acid (Z,Z)-, methyl ester (41.24 %), hexadecanoic acid, methyl Ester (27.39 %), 9 octadecenoic acid (Z)-,methyl ester (16.58%), methyl stearate (4.71%), ethyl linoleate (3.92%) and n-hexadecanoic acid (2.34%). According to these finding, Glycine max methanol extract is potential as anticancer and antimicrobial agents that is may be due to the identified bioactive compoundsby GC-MS analysis. Therefore, the phytochemicals of the plant may be responsible for the various pharmacological actions. © 2019, Advanced Scientific Research. All rights reserved.

Keywords

Antibacterial; Antifungal; GC-MS analysis; HepG2 cell line; Murine splenic cells; Soya bean; 1,2 benzenedicarboxylic acid,3 nitro; 9 octadecenoic acid (z) ,methyl ester; 9,12 octadecadienoic acid (z,z) , methyl ester; 9,12 octadecadienoyl chloride,(z,z); antiinfective agent; cytotoxic agent; glycine max seed extract; hexadecanoic acid, methyl ester; methyl Ester; methyl tetradecanoate; n hexadecanoic acid; palmitic acid; pentadecanoic acid, 14 methyl , methyl ester; plant extract; tetradecanoic acid, 12 methyl , methyl ester; unclassified drug; animal cell; antifungal activity; antimicrobial activity; Article; controlled study; cytotoxicity; enzyme linked immunosorbent assay; Hep-G2 cell line; human; human cell; IC50; mass fragmentography; minimum inhibitory concentration; mouse; nonhuman; phytochemistry; soybean; zone of inhibition

Citation Information

Scopus Citations: 6

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