Isolation and identification of certain phenolic compounds from salix mucronata leaf extracts and evaluation them as antimicrobial agents

Theodor Bilharz Research Institute

Bibliographic Information

Authors: El-Sayeda M.M.; Abdel-Azizb M.M.; Abo-Sedrac S.; Mohameda H.; Abdel-Lateefa E.E.-S.

Journal: Current Bioactive Compounds

Publisher: Bentham Science Publishers

Publication Date: 7 May 2019

Volume / Issue: Volume 15 / Issue 3

Pages: 360–366

ISSN: 15734072

DOI: 10.2174/1573407214666180829124509

Scopus: View on Scopus

Document Type: Article

Authors and Affiliations

El-Sayeda M.M., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt; Abdel-Azizb M.M., Department of Chemistry, Faculty of Science, Ain-Shams University, Cairo, Egypt; Abo-Sedrac S., Microbiology Department, National Research Center, Giza, Egypt; Mohameda H., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt; Abdel-Lateefa E.E.-S., Medicinal Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Background: In recent years, plant extracts are considered as an important source of many drug formulations for treatment of human beings from infection diseases. The objective of this study was to evaluate the antimicrobial activity of Salix mucronata leaves extracts and isolate their bioactive phytochemicals. Methods: The dry powder of Salix mucronata was extracted with different aqueous methanol concentrations. The 85% methanolic extract was further fractionated using different organic solvents. The antimicrobial activity of different extracts and fractions was evaluated. The most bioactive fractions were submitted for chromatographic isolation and structure elucidation of their phytochemicals using chromatographic and spectroscopic methods. Results: The ethyl acetate and the butanolic fractions derived from 85% MeOH extract gave a high antimicrobial activity with inhibition zones ranging between 10 mm and 26 mm and minimum inhibitory concentration (MIC) value of 8 mg/mL. While the butanolic fraction showed zones of inhibition ranging between 10 mm and 25 mm with MIC 8 mg/mL. Six compounds were isolated from ethyl acetate fraction and their structures were elucidated as; apigenin (1), quercetin (2), quercetrin (3), rhamnazin -3-O-β-D-glucopyranoside (4), Chrysoeriol-7-O-β-D-glucuronoid- 6″ -methyl ester (5), and tremuloidin (6). Also, five compounds were isolated from the butanolic fraction and their structures were elucidated as; kaempferol (7), luteolin (8), luteolin-3' - methoxy-4' - O-β-D- glucopyranoside (9), isorhamnetin -3-O-β -D-glucopyranoside (10) and salicin (11). Conclusion: The results of the present study showed that the ethyl acetate and the butanolic fractions contain high flavonoids and salicinoids compounds which may attribute to their potential as antimicrobial agents. © 2019 Bentham Science Publishers.

Keywords

Antimicrobial properties; Chromatography; Flavonoids; Phenolic compounds; Salicinoids; Salix mucronata; antibiotic agent; antifungal agent; apigenin; chrysoeriol 7 o beta dextro glucuronoid 6'' methyl ester; gentamicin; isorhamnetin 3 o beta dextro glucopyranoside; kaempferol; luteolin; luteolin 3' methoxy 4 o beta dextro glucopyranoside; nystatin; phenol derivative; quercetin; quercetrin; rhamnazin 3 beta dextro glucopyranoside; salicin; Salix extract; Salix mucronata extract; tremuloidin; unclassified drug; antibacterial activity; antibiotic sensitivity; antifungal activity; Article; Aspergillus niger; Bacillus subtilis; Candida albicans; controlled study; drug identification; drug isolation; drug structure; dry powder; Escherichia coli; minimum inhibitory concentration; nonhuman; plant leaf; spectroscopy; Staphylococcus aureus; willow; zone of inhibition

Citation Information

Scopus Citations: 1

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