Potentials of differentiated human cord blood-derived unrestricted somatic stem cells in treatment of liver cirrhosis

Theodor Bilharz Research Institute

Bibliographic Information

Authors: El Baz H.; Demerdash Z.; Kamel M.; Atta S.A.; Salah F.; Hassan S.; Hammam O.; Khalil H.; Bayoumi A.

Journal: Experimental and Clinical Transplantation

Publisher: Baskent University

Publication Date: April 2019

Volume / Issue: Volume 17 / Issue 2

Pages: 251–258

ISSN: 13040855

DOI: 10.6002/ect.2017.0249

Scopus: View on Scopus

PubMed: 30346265

Document Type: Article

Authors and Affiliations

El Baz H., The Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Demerdash Z., The Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Kamel M., The Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Atta S.A., The Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Salah F., The Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Hassan S., The Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt; Hammam O., The Pathology Department, Theodor Bilharz Research Institute, Giza, Egypt; Khalil H., The Pathology Department, Theodor Bilharz Research Institute, Giza, Egypt; Bayoumi A., The Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt

Abstract

Objectives: Liver transplantation is the well-known treatment for chronic liver diseases; however, post - operative complications and lack of donors continue to be limitations with this treatment. Investigating new modalities for treatment of chronic liver illness is a must. In the present study, we aimed to clarify the effects of an in vitro hepatocyte-differentiated human unrestricted somatic stem cell transplant as a new cellbased therapy in an experimental model of chronic liver failure. Materials and Methods: Human umbilical cord bloodderived unrestricted somatic stem cells were isolated, cultured, propagated, and characterized. Cells were directed to differentiate into hepatocyte-like cells. An animal model of carbon tetrachloride cirrhotic liver failure was prepared, and the human in vitro differentiated unrestricted somatic stem cells were transplanted into the experimental model. Animals that did not receive transplant served as the pathologic control group. Animals were euthanized 12 weeks after transplant, and liver functions and histopathology were assessed. Results: Compared with the pathologic control group, the transplant group showed improvements in levels of alanine aminotransferase, aspartate aminotransferase, albumin, and bilirubin. Histopathologic examination of the transplant group also showed improvements in hydropic degeneration and fibrosis. Conclusions: The use of unrestricted somatic stem cells, isolated and propagated from cord blood and then differentiated into hepatocyte-like cells, improved both fibrosis and normal function of cirrhotic livers. These cells could be considered as a line of cell-based therapy in cases of chronic liver disease. © Başkent University 2019.

Keywords

Cell-based therapy; Chronic liver disease; Adult Stem Cells; Animals; Biomarkers; Carbon Tetrachloride; Cell Differentiation; Cell Proliferation; Cells, Cultured; Chemical and Drug Induced Liver Injury; Cord Blood Stem Cell Transplantation; Fetal Blood; Hepatocytes; Humans; Liver; Liver Cirrhosis, Experimental; Liver Regeneration; Mice, Inbred BALB C; Phenotype; Time Factors; alanine aminotransferase; albumin; aspartate aminotransferase; bilirubin; CD34 antigen; Hermes antigen; Thy 1 membrane glycoprotein; biological marker; alanine aminotransferase blood level; albumin blood level; animal cell; animal experiment; animal model; animal tissue; Article; aspartate aminotransferase blood level; bilirubin blood level; cell count; cell culture; cell expansion; cell isolation; histopathology; in vitro study; liver cell; liver cirrhosis; liver fibrosis; liver function; mesenchymal stem cell; mouse; nonhuman; somatic cell; treatment outcome; treatment response; adult stem cell; animal; Bagg albino mouse; chemically induced; cytology; experimental liver cirrhosis; fetus blood; human; metabolism; pathology; procedures; time factor; toxic hepatitis; transplantation

Citation Information

Scopus Citations: 6

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