Molluscicidal effectiveness of Luo-Wei, a novel plant-derived molluscicide, against Oncomelania hupensis, Biomphalaria alexandrina and Bulinus truncatus

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Jia T.-W.; Wang W.; Sun L.-P.; Lv S.; Yang K.; Zhang N.-M.; Huang X.-B.; Liu J.-B.; Liu H.-C.; Liu R.-H.; Gawish F.A.; Habib M.R.; El-Emam M.A.; King C.H.; Zhou X.-N.

Journal: Infectious Diseases of Poverty

Publisher: BioMed Central Ltd.

Publication Date: 31 March 2019

Volume / Issue: Volume 8 / Issue 1

Article No.: 27

ISSN: 20955162

DOI: 10.1186/s40249-019-0535-7

Scopus: View on Scopus

PubMed: 31014390

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Jia T.-W., National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, 200025, China, Chinese Center for Tropical Diseases Research, Shanghai, 200025, China, WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, China, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, China, Communicable Diseases Cluster, World Health Organization Regional Office for Africa (WHO/AFRO), PO Box 06, Brazzaville, Congo; Wang W., Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasites and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, 214064, China; Sun L.-P., Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasites and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, 214064, China; Lv S., National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, 200025, China, Chinese Center for Tropical Diseases Research, Shanghai, 200025, China, WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, China, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, China; Yang K., Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasites and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, 214064, China; Zhang N.-M., Hubei Jinhaichao Science and Technology Co. Ltd, Wuhan, 430206, China; Huang X.-B., Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430079, China; Liu J.-B., Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430079, China; Liu H.-C., Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430079, China; Liu R.-H., School of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan, 430200, China; Gawish F.A., Department of Medical Malacology, Theodor Bilharz Research Institute (TBRI), Imbaba, Giza, 12411, Egypt; Habib M.R., Department of Medical Malacology, Theodor Bilharz Research Institute (TBRI), Imbaba, Giza, 12411, Egypt; El-Emam M.A., Department of Medical Malacology, Theodor Bilharz Research Institute (TBRI), Imbaba, Giza, 12411, Egypt; King C.H., Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH, United States, Schistosomiasis Consortium for Operational Research and Evaluation, University of Georgia, Athens, GA, United States; Zhou X.-N., National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, 200025, China, Chinese Center for Tropical Diseases Research, Shanghai, 200025, China, WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, China, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, 200025, China

Abstract

Background: Control of snail intermediate hosts has been proved to be a fast and efficient approach for interrupting the transmission of schistosomiasis. Some plant extracts have shown obvious molluscicidal activity, and a new compound Luo-Wei, also named tea-seed distilled saponin (TDS), was developed based on the saponins extracted from Camellia oleifera seeds. We aimed to test the molluscicidal activity of 4% TDS against the intermediate host snails in China and Egypt, and evaluate its environmental safety to non-target organisms. Methods: In the laboratory, Oncomelania hupensis, Biomphalaria alexandrina and Bulinus truncatus were exposed to 4% TDS, and the median lethal concentration (LC50) was estimated at 24, 48 and 72 h. In the field, snail mortalities were assessed 1, 2, 3 and 7 d post-immersion with 2.5 g/m3 4% TDS and 1, 3, 7 and 15 d post-spraying with 5 g/m2 4% TDS. In addition, the acute toxicity of 4% TDS to Japanese quail (Coturnix japonica), zebrafish (Brachydanio rerio) and freshwater shrimp (Macrobrachium nipponense) was assessed by estimations of LC50 or median lethal dose (LD50). Results: In the laboratory, the LC50 values of 4% TDS for O. hupensis were 0.701, 0.371 and 0.33 mg/L at 24, 48 and 72 h, respectively, and 4% TDS showed a 0.33 mg/L 24 h LC50 against B. alexandrina, and a 1.396 mg/L 24 h LC50 against B. truncatus. Across all study regions, the pooled mortalities of O. hupensis were 72, 86, 94 and 98% at 1, 2, 3 and 7 d, following field immersion of 4% TDS at a dose of 2.5 g/m3, and were 69, 77, 85 and 88% at 1, 3, 7 and 15 d, following field spraying at 5 g/m2, respectively. 4% TDS had moderate toxicity to Japanese quail (7 d LD50 > 60 mg/kg) and to shrimp (96 h LC50 = 6.28 mg/L; 95% CI: 3.53-11.2 mg/L), whereas its toxicity to zebrafish was high (96 h LC50 = 0.15 mg/L; 95% CI: 0.14-0.17 mg/L). Conclusions: 4% TDS is active against O. hupensis, B. alexandrina and B. truncatus under laboratory and field conditions, and it may be a candidate molluscicide of plant origin. © 2019 The Author(s).

Keywords

Biomphalaria alexandrina; Bulinus truncatus; Luo-Wei; Molluscicidal activity; Oncomelania hupensis; Plant-derived molluscicide; Schistosomiasis; Animals; Biomphalaria; Bulinus; China; Egypt; Lethal Dose 50; Molluscacides; Plant Extracts; Quail; Schistosoma; Snails; Toxicity Tests; luo wei; molluscacide; saponin derivative; tea seed distilled saponin; unclassified drug; plant extract; Article; Camellia oleifera; Coturnix japonica; ecotoxicology; LC50; mortality; nonhuman; plant seed; priority journal; Schistosoma japonicum; Schistosoma mansoni; snail; toxicity; Tursiops truncatus; zebra fish; animal; drug effect; LD50; parasitology; toxicity testing

Citation Information

Scopus Citations: 31

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