Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Atta A.H.; Mouneir S.M.; Nasr S.M.; Sedky D.; Mohamed A.M.; Atta S.A.; Desouky H.M.

Journal: Asian Pacific Journal of Tropical Biomedicine

Publisher: Wolters Kluwer Medknow Publications

Publication Date: 2019

Volume / Issue: Volume 9 / Issue 3

Pages: 98–108

ISSN: 22211691

DOI: 10.4103/2221-1691.254603

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Atta A.H., Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, P.O. box 12211, Egypt; Mouneir S.M., Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, P.O. box 12211, Egypt; Nasr S.M., Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth St., Dokki, Giza, 12622, Egypt; Sedky D., Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth St., Dokki, Giza, 12622, Egypt; Mohamed A.M., Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth St., Dokki, Giza, 12622, Egypt; Atta S.A., Immunology Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt; Desouky H.M., Department of Animal Reproduction and Artificial Insemination, National Research Centre, 33 Bohouth St., Dokki, Giza, 12622, Egypt

Abstract

Objective: To analyze the phytochemical constituents, and to explore potential protective effect of the methanol extract of Moringa oleifera (M. oleifera) seeds and Egyptian propolis, each alone or concurrently administered on acetic acid-induced ulcerative colitis in rats. Methods: Eight groups of 5 rats each were used: normal control group with distilled water, model group, two groups with M. oleifera seeds (100 and 200 mg/kg), two groups with propolis (50 and 100 mg/kg), one group with concurrent administration of both, and one group with prednisolone (reference drug). Macro-and microscopic picture, ulcer index and lesion scores, oxidative markers, inflammatory mediators, in vitro activity of the inflammatory enzymes and 1, 1-diphenyl-2-picrylhydrazyl free radicals scavenging activity were evaluated. The phytochemical constituents of both extracts were explored by GC-MS analysis. Results: Both treatments modulated the macro-and microscopic picture, decreased the ulcerative index, lesion score, oxidative markers and inflammatory mediators, and inhibited the COX-1 and COX-2 enzymes. Propolis appeared to be powerful free radicals scavenger. A powerful synergistic effect of both treatments in modulating the course of the disease was reported. GC-MS analysis of methanol extract of M. oleifera seeds and propolis revealed the presence of 50 and 34 compounds, respectively. Conclusions: M. oleifera seeds and propolis methanol extracts have modulated the course of acetic acid-induced ulcerative colitis. Moreover, both treatments induce a good synergistic effect against the disease. Isolation of the active constituents is recommended. © 2019 by the Asian Pacific Journal of Tropical Biomedicine.

Keywords

Moringa oleifera seeds; Phytochemical; Propolis; Ulcerative colitis; acetic acid; antiinflammatory agent; cyclooxygenase 1; cyclooxygenase 2; distilled water; malonaldehyde; methanol; Moringa oleifera extract; myeloperoxidase; nitrate; nitrite; prednisolone; tumor necrosis factor; animal experiment; animal model; animal tissue; antiinflammatory activity; antioxidant activity; Article; controlled study; DPPH radical scavenging assay; female; histopathology; in vitro study; male; mass fragmentography; Moringa oleifera; nonhuman; phytochemistry; plant seed; priority journal; rat; synergistic effect; ulcer index

Citation Information

Scopus Citations: 35

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