Prevalence of plasmid-mediated quinolone resistance in multidrug-resistant gram negative bacilli in Egypt

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abd ElSalam M.; Gamal D.; Said M.E.; Salem D.; Aitta A.A.; El Gamal M.S.

Journal: Biomedical and Pharmacology Journal

Publisher: Oriental Scientific Publishing Company

Publication Date: 3 December 2018

Volume / Issue: Volume 11 / Issue 4

Pages: 1927–1936

ISSN: 9746242

DOI: 10.13005/bpj/1565

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Abd ElSalam M., Departmentof Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt; Gamal D., Department of Microbiology, Theodor Bilharz Research Institute, Giza, Egypt; Said M.E., Department of Microbiology, Theodor Bilharz Research Institute, Giza, Egypt; Salem D., Department of Microbiology, Theodor Bilharz Research Institute, Giza, Egypt; Aitta A.A., Department of Microbiology, Theodor Bilharz Research Institute, Giza, Egypt; El Gamal M.S., Departmentof Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt

Abstract

Resistance to quinolone has increased significantly and one of the most reasons is plasmid-mediated quinolone resistance (PMQR). The aim of this study is to detect the prevalence of PMQR in multidrug-resistant (MDR) Gram negative bacilli and to characterize these resistance genes. A total of 420 Gram negative bacilli clinical isolates were collected from patients attending Misr children hospital. Isolates were identified by biochemical reactions, while antimicrobial susceptibility testingwas done by Kirby-Bauer disk diffusion method. Minimum inhibitory concentrations (MIC) of ciprofloxacin were detected by E-test, whereas combined test method was used to detect extended-spectrum β-lactamase (ESBL) production. QnrA, qnrB, and qnrS genes were determined by multiplex polymerase chain reaction (PCR). MDRGram negative bacilli represented 68% (268/420); most of them were recovered from blood culture specimens (21%).Among these MDR isolates21%(60/268) were ciprofloxacin resistant; with MICs >32µg/ ml in 95% of the isolates.ESBL production was detected in 11.7% of the studied isolates. The qnr genes were detected in 60%. QnrS and qnrB were the detected genes in 77.8% and 16.7% of the isolates respectively. Both qnrB and qnrS genes were determined simultaneously in 5.5%. QnrB gene was found alone in only one isolate (14.3%) that was ESBL-producer. The most MDR isolates were recovered from blood culture; this confirms the occurrence of these superbugs and their ability to cause life threatening infections. The prevalence of quinolone resistant Gram negative bacilli clinical isolates is high. The mostly prevalent PMQR gene is qnrS followed by qnrB. Published by Oriental Scientific Publishing Company © 2018

Keywords

ESBLs; Gram Negative Bacilli; Multiplex PCR; Plasmid-Mediated Quinolone Resistance; amikacin; amoxicillin plus clavulanic acid; ampicillin; cefoperazone; cefotaxime; ceftazidime; ceftriaxone; ciprofloxacin; extended spectrum beta lactamase; gentamicin; imipenem; quinolone; sulbactam; agar gel electrophoresis; antibiotic resistance; antibiotic sensitivity; Article; bacterial virulence; bacterium identification; bacterium isolate; bacterium isolation; blood culture; controlled study; disk diffusion; Enterobacter cloacae; epsilometer test; Escherichia coli; female; gene; genetic algorithm; Gram negative bacterium; human; Klebsiella pneumoniae; male; minimum inhibitory concentration; molecular size; multidrug resistance; multiplex polymerase chain reaction; nonhuman; phenotype; plasmid; plasmid mediated quinolone resistance; prevalence

Citation Information

Scopus Citations: 4

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