DNA vaccination using recombinant Schistosoma mansoni fatty acid binding protein (smFABP) gene

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Aly I.; ELnain G.; Hamad R.S.; Kilany M.; Ghramh H.A.; Alshehri A.; Dajem S.M.; Ibrahim E.H.

Journal: Experimental Parasitology

Publisher: Academic Press Inc.

Publication Date: November 2018

Volume / Issue: Volume 194

Pages: 53–59

ISSN: 144894

DOI: 10.1016/j.exppara.2018.09.018

Scopus: View on Scopus

PubMed: 30266572

Document Type: Article

Authors and Affiliations

Aly I., Parasitology Laboratory, Theodor Bilharz Research Institute, Imbaba, P.O. Box 30, Giza, Egypt; ELnain G., Parasitology Laboratory, Theodor Bilharz Research Institute, Imbaba, P.O. Box 30, Giza, Egypt, Natural Science, Mathan Science Program, University College, Abu Dhabi University, United Arab Emirates; Hamad R.S., Parasitology Laboratory, Theodor Bilharz Research Institute, Imbaba, P.O. Box 30, Giza, Egypt, Biological Science Department, King Faisal University, Saudi Arabia; Kilany M., Biology Department, Faculty of Sciences and Arts, King Khalid University, Dhahran Al Janoub, Saudi Arabia, Department of Microbiology, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt; Ghramh H.A., Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia, Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia, Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia; Alshehri A., Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia; Dajem S.M., Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia; Ibrahim E.H., Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia, Blood Products Quality Control and Research Department, National Organization for Research and Control of Biologicals, Cairo, Egypt

Abstract

Schistosomiasis is a fatal disease that has a negative impact on health and economics. Praziquantel (PZQ) is the drug of choice for schistosomiasis treatment, but it has no prophylactic effect; therefore, vaccination is an essential requirement in schistosomiasis control. This work was carried out to investigate the possible effect of DNA vaccination against Schistosoma mansoni infection using recombinant S. mansoni fatty acid binding protein (rsmFABP). The smFABP gene was cloned into the eukaryotic expression vector pcDNAI/Amp in order to obtain an smFABP-pcDNAI recombinant plasmid (DNA vaccine) and was used for the intramuscular DNA vaccination of out-bread Swiss albino mice prior to infection with S. mansoni cercariae. Infected groups, either DNA vaccinated or unvaccinated, were treated with PZQ at week 6 post-infection. After 8 weeks post-infection, all mouse groups were sacrificed and parasitological, immunological and histopathological parameters were studied. DNA vaccinated mice showed a high titer of anti-smFABP-IgG antibodies and acquired significant protection (74.2%, p < 0.01) against S. mansoni infection, with a reduction in ova and granuloma counts. DNA vaccinated and PZQ treated animals had higher titers of anti-smFABP-IgG antibodies and decreased (87%, P < 0.001) parenchymal granulomas compared to the DNA vaccinated PZQ untreated group. Infected mice, either non DNA vaccinated or vaccinated, had very high collagen content and fibrous granulomas (74%) compared to the PZQ treated group (10.3% fibrous granuloma) and PZQ treated + DNA vaccinated group (0% fibrous granuloma). In conclusion, DNA vaccination had protective and anti-pathological effects in naive mice and greatly improved the pathological status in PZQ-treated animals, suggesting an immunological and pathological modulating effect of PZQ treatment. © 2018 Elsevier Inc.

Keywords

Cloning; DNA vaccination; Schistosoma mansoni; Schistosomiasis control; smFABP; Amino Acid Sequence; Animals; Biomphalaria; Cricetinae; Fatty Acid-Binding Proteins; Female; Helminth Proteins; Liver; Mesocricetus; Mice; Schistosomiasis mansoni; Vaccines, DNA; collagen; fatty acid binding protein; immunoglobulin G antibody; praziquantel; Schistosoma vaccine; DNA vaccine; helminth protein; animal experiment; animal model; animal tissue; antibody response; antibody titer; Article; controlled study; DNA immunization; expression vector; liver granuloma; mouse; nonhuman; priority journal; recombinant plasmid; tissue egg count; worm burden; animal; chemistry; genetics; hamster; immunology; pathology

Citation Information

Scopus Citations: 4

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