Detection of antimicrobial resistance genes of Helicobacter pylori strains to clarithromycin, metronidazole, amoxicillin and tetracycline among Egyptian patients

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Diab M.; El-Shenawy A.; El-Ghannam M.; Salem D.; Abdelnasser M.; Shaheen M.; Abdel-Hady M.; El-Sherbini E.; Saber M.

Journal: Egyptian Journal of Medical Human Genetics

Publisher: Egyptian Society of Human Genetics

Publication Date: October 2018

Volume / Issue: Volume 19 / Issue 4

Pages: 417–423

ISSN: 11108630

DOI: 10.1016/j.ejmhg.2018.01.004

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access

Authors and Affiliations

Diab M., Department of Microbiology, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt; El-Shenawy A., Department of Microbiology, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt; El-Ghannam M., Department of Gastroentrology and Hepatology, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt; Salem D., Department of Microbiology, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt; Abdelnasser M., Department of Medical Microbiology and Immunology, Faculty of Medicine Al-Azhar University, Egypt; Shaheen M., Department of Biochemistry, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt; Abdel-Hady M., Department of Medical Microbiology and Immunology, Faculty of Medicine Al-Azhar University, Egypt; El-Sherbini E., Department of Microbiology, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt; Saber M., Department of Biochemistry, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt

Abstract

Background: Antibiotic resistance of Helicobacter pylori (H. pylori) treatment is on the rise, and is affecting the efficacy of current used therapeutic regimens. Aim: We aimed to enhance the understanding of antimicrobial resistance rates of H. pylori strains recovered from patients at Theodor Bilharz Research Institute Hospital in Egypt, as a mandatory step before starting treatment. Subjects and methods: Mutant genes conferring metronidazole, amoxicillin, clarithromycin, and tetracycline resistance were detected in 60 H. pylori strains recovered from patients who underwent upper endoscopic examination. Patients were considered to be infected with H. pylori when rapid urease test and detection of 16S rRNA in gastric biopsies recorded positive. Molecular detection of resistant genes to metronidazole (rdx gene) and amoxicillin (pbp1A gene) was carried out by conventional PCR followed by sequencing of PCR products. While detection of 23S rRNA gene conferring clarithromycin resistance and detection of 16S rRNA mutation gene conferring tetracycline resistance were carried out by real-time PCR. Results: H. pylori resistance rates to metronidazole, and amoxicillin were 25% and 18.3% respectively. While for clarithromycin and tetracycline, point mutations in 23S rRNA types A2142G and A2143G and in 16S rRNA of H. pylori were assessed by real time PCR assay respectively. Resistance mutant genes were found to be 6.7% of clarithromycin and 1.7% of tetracycline. Combined resistance rates to metronidazole and amoxicillin was (11.6%) followed by metronidazole and clarithromycin (5%), while patterns of clarithromycin and amoxicillin (1.6%), metronidazole, clarithromycin and amoxicillin (1.6%) were revealed. Conclusion: Data concerning antimicrobial resistance genes play an important role in empiric treatment of H. pylori infection. According to our results, H. pylori resistance to metronidazole and amoxicillin was relatively high. Clarithromycin is still a good option for first line anti-H. pylori treatment. Combined resistant strains are emerging and may have an effect on the combination therapy. © 2018 Ain Shams University

Keywords

Antimicrobial resistance; Clarithromycin resistance; Gene mutations; H. pylori; Rdx gene; Tetracycline resistance; amoxicillin; clarithromycin; metronidazole; RNA 16S; RNA 23S; tetracycline; adolescent; adult; aged; antibiotic resistance; Article; bacterial gene; bacterial strain; bacterium detection; Egypt; Egyptian; female; Helicobacter pylori; human; human tissue; major clinical study; male; point mutation; real time polymerase chain reaction; stomach biopsy

Citation Information

Scopus Citations: 22

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