Colonic stem cells expression of Lgr5 and CD133 proteins as predictive markers in colorectal cancer among Egyptian patients

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Rosiq S.; Hammam O.; Abdelalim A.; Anas A.; Khalil H.; Amer M.

Journal: Open Access Macedonian Journal of Medical Sciences

Publisher: Open Access Macedonian Journal of Medical Sciences

Publication Date: 7 June 2018

Volume / Issue: Volume 6 / Issue 6

Pages: 968–974

ISSN: 18579655

DOI: 10.3889/oamjms.2018.208

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

Rosiq S., Tropical Medicine Department, Al Azhar University, Cairo, Egypt; Hammam O., Pathology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Abdelalim A., Tropical Medicine Department, Al Azhar University, Cairo, Egypt; Anas A., Hepato-Gastroenterology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Khalil H., Pathology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt; Amer M., Tropical Medicine Department, Nasser Institute Hospital, Cairo, Egypt

Abstract

AIM: Colorectal cancer is the fourth common tumour in Egypt after lymphoid, breast and urinary tumours. The study aims to assess the expression of Lgr5 and CD133 in pre-malignant (adenomatous polyps and IBD), malignant colorectal lesions and normal colonic mucosa by immunohistochemical staining. MATERIAL AND METHODS: This prospective study was done on 100 patients presenting with colonic symptoms, patients were divided into four groups; group I including 20 patients in the control group, group II including 20 ulcerative colitis (U.C) patients, group III including 20 patients with adenomatous polyps and group IV including 40 patients with colorectal cancer (CRC). RESULTS: Lgr5 and CD133 expression was significantly higher in carcinoma than in adenomas, IBD and normal mucosa (P < 0.001). Lrg5 and CD133 was positively correlated with histological grade (P = 0.001), depth of invasion (P = 0.001), lymph node metastasis (P < 0.001), distant metastasis (P < 0.004) and TNM stage (P < 0.001). CONCLUSION: Role of Lgr5 and CD133 as stem cell marker was expressed and presented with different expression in the normal colonic mucosa, adenoma and CRC and showed increased expression in an advanced stage of CRC. This may suggest its possible involvement in colorectal tumorigenesis and invasion. © 2018 Saed Rosiq, Olfat Hammam, Ahmed Abdelalim, Amgad Anas, Heba Khalil, Mosbah Amer.

Keywords

CD133; Colonic cancer; IHC; Lgr5; Polyp; CD133 antigen; protein; protein Lgr5; tumor marker; unclassified drug; adenomatous polyp; adult; Article; cancer patient; cancer staging; case control study; clinical feature; colon carcinogenesis; colon mucosa; colonoscopy; colorectal adenoma; colorectal carcinoma; colorectal polyp; controlled study; correlation analysis; distant metastasis; Egyptian; female; gastrointestinal symptom; histopathology; human; human tissue; immunohistochemistry; intestinal stem cell; lymph node metastasis; major clinical study; male; middle aged; precancer; predictive value; prospective study; protein expression; tumor invasion; ulcerative colitis

Citation Information

Scopus Citations: 6

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