HPLC-ESI-MS Characterization of Certain Polyphenolic Compounds of Carica papaya LFruit Extracts and Evaluation of Their Potential Against Murine Schistosomiasis mansoni

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Abdel-Lateef E.E.-S.; Rabia I.A.; El-Sayed M.M.; Abdel-Hameed E.-S.S.

Journal: Drug Research

Publisher: Georg Thieme Verlag

Publication Date: 10 April 2018

Volume / Issue: Volume 68 / Issue 9

Pages: 521–528

ISSN: 21949379

DOI: 10.1055/a-0592-6550

Scopus: View on Scopus

PubMed: 29635673

Document Type: Article

Authors and Affiliations

Abdel-Lateef E.E.-S., Medicinal Chemistry Laboratory, Theodor Bilharz Research Institute, P.O. Box 30, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Rabia I.A., Parasitology Laboratory, Theodor Bilharz Research Institute, Giza, Egypt; El-Sayed M.M., Medicinal Chemistry Laboratory, Theodor Bilharz Research Institute, P.O. Box 30, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Abdel-Hameed E.-S.S., Medicinal Chemistry Laboratory, Theodor Bilharz Research Institute, P.O. Box 30, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt, Chemistry Department, Faculty of Science, Taif University, Jeddah, Saudi Arabia

Abstract

The in vivo antischistosomal activities of Carica papaya L. extracts were evaluated and the characterization of the active secondary metabolites of the defatted methanolic extract was performed using HPLC-ESI-MS. The plant fruit powders were extracted with 85% methanol and fractionated using organic solvents. The in vivo antischistosomal effects of the methanolic extracts and its fractions, as well as the assessment of the relationship between the antischistosomal activity of these plant extracts and oxidative stress, was determined. In addition, the defatted methanolic extract was characterized by HPLC-ESI-MS analysis. The number of worms, ova, and the Oogram pattern displayed typical Schistosoma mansoni pathology 8 weeks after infection in mice. Treatment of the infected group with the defatted methanolic extracts significantly decreased worm burden, immature ova and mature ova, while increasing the percentage of dead ova in vivo. The butanol fraction was the most effective fraction reducing worm burden by 77%, ova count in the intestine by 76% and in the liver by 80%, and significantly decreased immature and mature ova (P <0.001) compared to the infected group. Additionally, the defatted methanolic extracts improved the reduced glutathione and malondialdehyde levels in hepatic tissues in the treated groups compared to the infected group. The HPLC-ESI-MS analysis of the Carica papaya defatted methanolic extract revealed the presence of several polyphenolic compounds. Carica papaya fruit extracts are rich with phenolic acids and flavonoids and show a significant effect against S. mansoni infections which may be used alternative to PZQ as anti-schistosomal drug against schistosomiasis. © 2018 Georg Thieme Verlag KG Stuttgart - New York.

Keywords

Carica papaya; HPLC-ESI-MS; polyphenolic compounds; Schistosoma mansoni; Animals; Antioxidants; Carica; Chromatography, High Pressure Liquid; Fruit; Male; Mice; Plant Extracts; Schistosomiasis mansoni; Schistosomicides; Spectrometry, Mass, Electrospray Ionization; 4 hydroxybenzoic acid o glucuronide; antischistosomal agent; Carica papaya extract; diosmetin 7 o rutinoside; flavonoid; glutathione; malonaldehyde; methanol; polyphenol derivative; praziquantel; protocatechuic acid; protocatechuic acid di o hexoside; protocatechuic di o hexosyl glucuronide; reactive oxygen metabolite; rosmarinic acid; rutoside; trihydroxy octadecenoic acid; unclassified drug; antioxidant; plant extract; animal experiment; animal model; animal tissue; anthelmintic activity; antioxidant activity; Article; electrospray mass spectrometry; high performance liquid chromatography; in vivo study; intestine; lipid peroxidation; liver; liver tissue; mouse; nonhuman; oxidative stress; worm burden; animal; chemistry; procedures

Citation Information

Scopus Citations: 5

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