High-performance liquid chromatography-fingerprint analyses, in vitro cytotoxicity, antimicrobial and antioxidant activities of the extracts of two cestrum species growing in Egypt

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Nasr S.; Ghareeb M.; Mohamed M.; Elwan N.; Abdel-Aziz A.E.-W.; Abdel-Aziz M.

Journal: Pharmacognosy Research

Publisher: Medknow Publications

Publication Date: 2018

Volume / Issue: Volume 10 / Issue 2

Pages: 173–180

ISSN: 9748490

DOI: 10.4103/pr.pr_145_17

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Hybrid Gold Open Access

Authors and Affiliations

Nasr S., Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, P.O. 30, Warrak El-Hadar, Imbaba, Giza, 12411, Egypt; Ghareeb M., Medicinal Chemistry Department, Theodor Bilharz Research Institute, P.O. 30, Kornaish El-Nile, Warrak El-Hadar, Imbaba, 12411, Egypt; Mohamed M., Medicinal Chemistry Department, Theodor Bilharz Research Institute, P.O. 30, Kornaish El-Nile, Warrak El-Hadar, Imbaba, 12411, Egypt; Elwan N., Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt; Abdel-Aziz A.E.-W., Medicinal Chemistry Department, Theodor Bilharz Research Institute, P.O. 30, Kornaish El-Nile, Warrak El-Hadar, Imbaba, 12411, Egypt; Abdel-Aziz M., Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, El-Bohouth Street 33, Dokki-Giza, Egypt

Abstract

Background: Cancer diseases and microbial resistance are serious health disorders associated with oxidative stress and infectious diseases. Their risks can be reducing via using polyphenols-rich plants. Methodology: Different solvent extracts from two Cestrum species (Cestrum nocturnum and Cestrum elegans) were evaluated for their biological and chemical activities. Also, the chemical profiles of the most promising extracts were investigated via high-performance liquid chromatography (HPLC)-fingerprint analyses. Results: The tested extracts showed weak to moderate cytotoxicity against Vero cell line with IC50values ranged from 133.67 μg/ml to 57.634 μg/ml. The only noncytotoxic extractive fraction was the dichloromethane extract of C. elegans leaves with an IC50value of 204.732 μg/ml, while the most toxic extract was the ethyl acetate extract of C. elegans flowers with an IC50value of 19.22 μg/ml. The antimicrobial activity results revealed that the n-BuOH extract of C. nocturnum was the most active against four tested microbial strains with inhibition zones (10-13 mm). Also, the water and n-BuOH extracts of C. elegans leave exhibited moderate activities with inhibition zones (7-9 mm), while for C. elegans flowers both of water and methanol extracts showed strong activities (9-14 mm). In the 2,2'-diphenyl-1-picrylhydrazyl assay, the most active fraction was EtOAc with IC50values of 100.52 μg/ml and 64.40 μg/ml for C. elegans leaves and flowers respectively, while for C. nocturnum the most active fraction was methanol with an IC50value of 161.16 μg/ml, all relative to 7.60 μg/ml of ascorbic acid. HPLC-fingerprint analyses revealed that the major identified compounds in the ethyl acetate extract of C. elegans flowers are caffeic acid, coumaric acid, vanillin, and rutin, while for the n-butanol extract of C. nocturnum leaves are coumaric acid and vanillin. Conclusion: The obtained results revealed that the two species can be used as natural sources of antioxidant compounds with low cytotoxic effect on the mammalian cell line. Abbreviations Used: HPLC: High-performance liquid chromatography; IC50: Median inhibitory concentration; DPPH: 2,2'-Diphenyl-1-picrylhydrazyl; ATCC: American Type Culture Collection; ECACC: European Collection of Animal Cell Cultures; HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; GM: Growth medium; EDTA: Ethylene diamine tetra acetic acid; MM: Maintenance media; ELISA: Enzyme-linked immuno-sorbent assay; G+ve: Gram-positive; G-ve: Gram-negative; IP: Inhibition percentage; RP-HPLC: Reversed phase-high performance liquid chromatography; DAD: Diode array detection; NCI: National Cancer Institute; DCM: Dichloro methane. © 2018 Pharmacognosy Research | Published by Wolters Kluwer - Medknow.

Keywords

2 2'-Diphenyl-1-picrylhydrazyl; antimicrobial; Cestrum species; cytotoxicity; high-performance liquid chromatography-fingerprint analyses; Solanaceae; acetic acid ethyl ester; ascorbic acid; butanol; caffeic acid; catechin; Cestrum elegans extract; Cestrum nocturnum extract; cinnamic acid; coumaric acid; dichloromethane; gallic acid; griseofulvin; methanol; penicillin G; plant extract; quercetin; rutoside; syringic acid; unclassified drug; vanillin; water; animal cell; antimicrobial activity; antioxidant activity; Article; biological activity; Cestrum; Cestrum elegans; Cestrum nocturnum; chemical analysis; controlled study; DPPH radical scavenging assay; drug cytotoxicity; Egypt; flower; high performance liquid chromatography; IC50; nonhuman; plant leaf; Vero cell line

Citation Information

Scopus Citations: 18

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