Advantages of bioconjugated silica-coated nanoparticles as an innovative diagnosis for human toxoplasmosis

Theodor Bilharz Research Institute

Bibliographic Information

Authors: Aly I.; Taher E.E.; EL nain G.; EL Sayed H.; Mohammed F.A.; Hamad R.S.; Bayoumy E.M.

Journal: Acta Tropica

Publisher: Elsevier B.V.

Publication Date: January 2018

Volume / Issue: Volume 177

Pages: 19–24

ISSN: 0001706X

DOI: 10.1016/j.actatropica.2017.09.024

Scopus: View on Scopus

PubMed: 28964770

Document Type: Article

Authors and Affiliations

Aly I., Parasitology Laboratory, Theodor Bilharz Research Institute, Giza, Egypt; Taher E.E., Clinical Parasitology Department, Research Institute of Ophthalmology, Giza, Egypt; EL nain G., Parasitology Laboratory, Theodor Bilharz Research Institute, Giza, Egypt, Natural Sciences, Math and Science Program, University College, Abu Dhabi University, Saudi Arabia; EL Sayed H., Ph.D. of immunology and parasitology, Egypt; Mohammed F.A., Parasitology Department, Faculty of Medicine, Zagazig University, Egypt; Hamad R.S., Department of Biological Sciences, College of science, King Faisal University, Saudi Arabia, Central Lab, Theodor Bilharz Research Institute, Giza, Egypt; Bayoumy E.M., Hydrobiology Department, National Research Centre, Dokki, Giza, Egypt, Biology Dept., Girls Science College, Imam Abdulrahman Bin Faisal University, Saudi Arabia, Biology Dept., Science Girls College. - Imam Abdulrahman Bin Faisal University, Saudi Arabia

Abstract

Nanotechnology is a promising arena for generating new applications in Medicine. To successfully functionalised nanoparticles for a given biomedical application, a wide range of chemical, physical and biological factors have to be taken into account. Silica-coated nanoparticles, (SiO2NP) exhibit substantial diagnostic activity owing to their large surface to volume ratios and crystallographic surface structure. This work aimed to evaluate the advantage of bioconjugation of SiO2NP with PAb against Toxoplasma lyzate antigen (TLA) as an innovative diagnostic method for human toxoplasmosis. This cross-sectional study included 120 individuals, divided into Group I: 70 patients suspected for Toxoplasma gondii based on the presence of clinical manifestation. Group II: 30 patients harboring other parasites than T. gondii Group III: 20 apparently healthy individuals free from toxoplasmosis and other parasitic infections served as negative control. Detection of circulating Toxoplasma antigen was performed by Sandwich ELISA and Nano-sandwich ELISA on sera and pooled urine of human samples. Using Sandwich ELISA, 10 out of 70 suspected Toxoplasma-infected human serum samples showed false negative and 8 out of 30 of other parasites groups were false positive giving 85.7% sensitivity and 84.0% specificity, while the sensitivity and specificity were 78.6% and 70% respectively in urine samples. Using Nano-Sandwich ELISA, 7 out of 70 suspected Toxoplasma-infected human samples showed false negative results and the sensitivity of the assay was 90.0%, while 4 out of 30 of other parasites groups were false positive giving 92.0% specificity, while the sensitivity and specificity were 82.6% and 80% respectively in urine samples. In conclusion, our data demonstrated that loading SiO2 nanoparticles with pAb increased the sensitivity and specificity of Nano-sandwich ELISA for detection of T.gondii antigens in serum and urine samples, thus active (early) and light infections could be easily detected. © 2017 Elsevier B.V.

Keywords

Diagnoses; Human toxoplasmosis; Nanotechnology; Toxoplasma gondii; Adult; Antigens, Protozoan; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G; Nanoparticles; Pregnancy; Sensitivity and Specificity; Silicon Dioxide; Toxoplasma; Toxoplasmosis; Young Adult; parasite antigen; polyclonal antibody; silica nanoparticle; Toxoplasma lyzate antigen; unclassified drug; nanoparticle; antigen; parasitic disease; protist; silica; antigen detection; Article; blood analysis; conjugation; controlled study; cross-sectional study; enzyme linked immunosorbent assay; false negative result; false positive result; human; major clinical study; polyacrylamide gel electrophoresis; urinalysis; chemistry; immunology

Citation Information

Scopus Citations: 20

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