Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients

Theodor Bilharz Research Institute

Bibliographic Information

Authors: El-Shenawy A.; Diab M.; Shemis M.; El-Ghannam M.; Salem D.; Abdelnasser M.; Shahin M.; Abdel-Hady M.; El-Sherbini E.; Saber M.

Journal: Egyptian Journal of Medical Human Genetics

Publisher: Egyptian Society of Human Genetics

Publication Date: October 2017

Volume / Issue: Volume 18 / Issue 4

Pages: 365–371

ISSN: 11108630

DOI: 10.1016/j.ejmhg.2017.04.003

Scopus: View on Scopus

Document Type: Article

Access: All Open Access; Gold Open Access; Green Open Access

Authors and Affiliations

El-Shenawy A., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Diab M., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Shemis M., Biochemistry Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; El-Ghannam M., Gastroentrology and Hepatology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Salem D., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Abdelnasser M., Medical Microbiology and Immunology Department, Faculty of Medicine Al-Azhar University, Egypt; Shahin M., Biochemistry Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Abdel-Hady M., Medical Microbiology and Immunology Department, Faculty of Medicine Al-Azhar University, Egypt; El-Sherbini E., Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt; Saber M., Biochemistry Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt

Abstract

Background Helicobactor pylori (H. pylori) virulence markers would be useful to predict peptic ulcer disease (PUD) or gastric cancer. Aim In Egypt, since inadequate data are present regarding H. pylori virulence–related genes in different age group patients with gastro-duodenal diseases, it becomes crucial to study the clinical status of cagA, vacA and iceA1 genotypes of H. pylori strains recovered from patients with dyspepsia. Subjects and methods The study included 113 dyspeptic patients who were exposed to upper gastrointestinal endoscopic examination. Four antral biopsies were obtained from each patient for the analysis of H. pylori infection by rapid urease test and detection of 16S rRNA. Results Sixty (53.1%) patients were confirmed to be infected with H. pylori. Upon endoscopy, gastritis was revealed in 27 patients (45%) and10 patients (16.7%) had PUD. Of the 60 H. pylori strains, 39 (65%) had at least one virulence gene. Six different genotypic forms were recognized; vacA (9/60), iceA1 (1/60), vacA/cagA (7/60), vacA/iceA1 (13/60), vacA/cagA/iceA1 (8/60) only one of cagA/iceA type and we could not detect cagA. The overall vacA, iceA1and cagA genes identified were 61.6%, 38.8%, 26.6% respectively, by PCR-based molecular testing. The vacA gene status was highly significant related to gastritis patient (P ≤ 0.036). The vacA s1m1 and s2m2 alleles were significantly found in 50% of H. pylori infected patients with PUD and with gastritis 57.1% respectively (P ≤ 0.01). Conclusion In conclusion, the main genotype combinations in the studied Egyptian patients were; vacAs2m2/iceA1, vacAs1m1/cagA, mostly associated with gastritis, and vacAs1/cagA/icA, mainly in PUD. The less virulent (s2, s2m2) H. pylori genotypes were found in patients aged over 43 years. © 2017 Ain Shams University

Keywords

cagA; Gastric biopsies; Helicobactor pylori; icA1; PCR–based genotyping; Peptic ulcer disease; vacA; bacterial DNA; bacterial protein; bacterial RNA; CagA protein; iceA1 protein; RNA 16S; unclassified drug; vacuolating toxin; adolescent; adult; aged; allele; Article; bacterial virulence; bacterium detection; clinical outcome; controlled study; DNA extraction; dyspepsia; Egypt; Egyptian; esophagitis; esophagus varices; gastritis; gastroesophageal reflux; gastrointestinal endoscopy; gene identification; genetic association; genotype; heartburn; Helicobacter infection; Helicobacter pylori; hiatus hernia; human; major clinical study; nonhuman; peptic ulcer; rapid urease test; stomach antrum prolapse; stomach biopsy; stomach cancer; stomach disease; vomiting

Citation Information

Scopus Citations: 21

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